培养的心肌细胞单层中的爆发性钙转子
Bursting calcium rotors in cultured cardiac myocyte monolayers.
作者信息
Bub G, Glass L, Publicover N G, Shrier A
机构信息
Department of Physiology, McGill University, 3655 Drummond Street, Montreal, QC Canada, H3G 1Y6.
出版信息
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10283-7. doi: 10.1073/pnas.95.17.10283.
Abstract
Rotating waves (rotors) of cellular activity were observed in nonconfluent cultures of embryonic chick heart cells by using a macroscopic imaging system that detected fluorescence from intracellular Ca2+. Unlike previous observations of rotors or spiral waves in other systems, the rotors did not persist but exhibited a repetitive pattern of spontaneous onset and offset leading to a bursting rhythm. Similar dynamics were observed in a cellular automaton model of excitable media that incorporates spontaneous initiation of activity, and a decrease of excitability as a consequence of rapid activity (fatigue). These results provide a mechanism for bursting dynamics in normal and pathological biological processes.
摘要
通过使用一种能检测细胞内钙离子荧光的宏观成像系统，在未汇合的胚胎鸡心脏细胞培养物中观察到了细胞活动的旋转波（转子）。与之前在其他系统中对转子或螺旋波的观察不同，这些转子并非持续存在，而是呈现出自发出现和消失的重复模式，从而导致一种爆发性节律。在一个包含活动自发起始以及由于快速活动（疲劳）导致兴奋性降低的可兴奋介质细胞自动机模型中，也观察到了类似的动力学现象。这些结果为正常和病理生物学过程中的爆发性动力学提供了一种机制。
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