番茄中的基因工程广谱抗病性
Genetically engineered broad-spectrum disease resistance in tomato.
作者信息
Oldroyd G E, Staskawicz B J
机构信息
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10300-5. doi: 10.1073/pnas.95.17.10300.
Abstract
Resistance in tomato to the bacterial pathogen Pseudomonas syringae pathovar tomato requires Pto and Prf. Mutations that eliminate Prf show a loss of both Pto resistance and sensitivity to the organophosphate insecticide fenthion, suggesting that Prf controls both phenotypes. Herein, we report that the overexpression of Prf leads to enhanced resistance to a number of normally virulent bacterial and viral pathogens and leads to increased sensitivity to fenthion. These plants express levels of salicylic acid comparable to plants induced for systemic acquired resistance (SAR) and constitutively express pathogenesis related genes. These results suggest that the overexpression of Prf activates the Pto and Fen pathways in a pathogen-independent manner and leads to the activation of SAR. Transgene-induced SAR has implications for the generation of broad spectrum disease resistance in agricultural crop plants.
摘要
番茄对细菌性病原菌丁香假单胞菌番茄致病变种的抗性需要Pto和Prf。消除Prf的突变会导致Pto抗性丧失以及对有机磷杀虫剂倍硫磷敏感，这表明Prf控制这两种表型。在此，我们报道Prf的过表达导致对多种通常具有毒性的细菌和病毒病原体的抗性增强，并导致对倍硫磷的敏感性增加。这些植物中水杨酸的表达水平与诱导产生系统获得性抗性（SAR）的植物相当，并组成型表达病程相关基因。这些结果表明，Prf的过表达以病原体非依赖的方式激活Pto和Fen途径，并导致SAR的激活。转基因诱导的SAR对农作物广谱抗病性的产生具有重要意义。
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