Akashi T, Aoki T, Ayabe S
Department of Applied Biological Science, Nihon University, Fujisawa, Kanagawa, Japan.
FEBS Lett. 1998 Jul 17;431(2):287-90. doi: 10.1016/s0014-5793(98)00781-9.
The microsome of insect cells expressing CYP Ge-5 (CYP93B1), a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), catalyzed the formation of [14C]licodione and [14C]2-hydroxynaringenin from (2S)-[14C]liquiritigenin and (2S)-[14C]naringenin, respectively. On acid treatment, the products were converted to 14C-labeled 7,4'-dihydroxyflavone and apigenin. Eriodictyol was also converted to luteolin by the reaction with the microsome of yeast expressing CYP93B1 and subsequent acid treatment. CYP93B1 was thus shown to encode (2S)-flavanone 2-hydroxylase, which has previously been designated to licodione synthase and flavone synthase II depending on the substrates employed.
表达甘草（Glycyrrhiza echinata L.）细胞色素P450 cDNA CYP Ge-5（CYP93B1）的昆虫细胞微粒体，分别催化（2S）-[14C]甘草素和（2S）-[14C]柚皮素形成[14C]甘草二酮和[14C]2-羟基柚皮素。经酸处理后，产物转化为14C标记的7,4'-二羟基黄酮和芹菜素。圣草酚与表达CYP93B1的酵母微粒体反应并随后经酸处理后也转化为木犀草素。因此表明CYP93B1编码（2S）-黄烷酮2-羟化酶，根据所使用的底物，该酶以前被指定为甘草二酮合酶和黄酮合酶II。
