Gordon L K, Eggena M, Holland G N, Weisz J M, Braun J
UCLA Ocular Inflammatory Disease Center, USA.
J Clin Immunol. 1998 Jul;18(4):264-71. doi: 10.1023/a:1027333822801.
A pANCA autoantibody (antineutrophil cytoplasmic antibody, perinuclear pattern) has been described in uveitis patients, but its correlation with systemic illnesses and the specific type of pANCA has not been defined. The goals of this study were to determine the (1) frequency of pANCA autoantibodies in uveitis, (2) systemic associations in the pANCA + uveitis patients, and (3) type of pANCA antigen recognized by the uveitis-associated autoantibody. Serum was obtained from 59 patients with anterior uveitis or panuveitis and from nonuveitis controls. A detailed medical and family history was obtained from each subject at the time of phlebotomy. Sera were screened by neutrophil ELISA to determine the frequency of ANCA positivity. Immunofluorescence assays were then used to differentiate cANCA from pANCA. The specificity of the pANCA + antibodies was further characterized by DNase 1 sensitivity and granule antigen ELISAs. ANCA antibodies were detected in 29% of all patients with panuveitis or anterior uveitis. In 41% of these ANCA + patients, serum antibody detected a perinuclear antigen that was sensitive in all cases to DNase 1. The majority of pANCA + uveitis patients were either HLA-B27 positive or had systemic evidence of immune-mediated diseases. Two pANCA + patients had no medical or family history of other immune-mediated diseases. This study identifies a subset of uveitis patients distinguished by expression of a specific pANCA marker antibody. The characteristics of this antibody are similar to the pANCA antibody present in most patients with ulcerative colitis. Expression of the pANCA autoantibody in uveitis patients is a susceptibility marker for other immune-mediated diseases.
葡萄膜炎患者中已发现存在一种核周型抗中性粒细胞胞浆抗体（pANCA），但它与全身性疾病的相关性以及pANCA的具体类型尚未明确。本研究的目的是确定：（1）葡萄膜炎患者中pANCA自身抗体的频率；（2）pANCA阳性葡萄膜炎患者的全身性关联；（3）葡萄膜炎相关自身抗体识别的pANCA抗原类型。收集了59例前葡萄膜炎或全葡萄膜炎患者以及非葡萄膜炎对照者的血清。在采血时获取了每位受试者详细的病史和家族史。通过中性粒细胞酶联免疫吸附测定（ELISA）筛查血清以确定ANCA阳性的频率。然后使用免疫荧光测定法区分胞浆型抗中性粒细胞胞浆抗体（cANCA）和核周型抗中性粒细胞胞浆抗体（pANCA）。通过脱氧核糖核酸酶1（DNase 1）敏感性和颗粒抗原ELISA进一步鉴定pANCA阳性抗体的特异性。在所有全葡萄膜炎或前葡萄膜炎患者中，29%检测到ANCA抗体。在这些ANCA阳性患者中，41%的血清抗体检测到一种核周抗原，所有病例中该抗原对DNase 1均敏感。大多数pANCA阳性葡萄膜炎患者要么人类白细胞抗原B27（HLA - B27）呈阳性，要么有免疫介导疾病的全身性证据。两名pANCA阳性患者没有其他免疫介导疾病的病史或家族史。本研究确定了一部分以特定pANCA标记抗体表达为特征的葡萄膜炎患者。该抗体的特征与大多数溃疡性结肠炎患者体内存在的pANCA抗体相似。葡萄膜炎患者中pANCA自身抗体的表达是其他免疫介导疾病的易感性标志物。
