Frenzilli G, Lori A, Panasiuk G, Ferdeghini M, Barale R
Dipartimento di Scienze dell'Uomo e dell'Ambiente, Università di Pisa, Italy.
Mutat Res. 1998 Jul 8;415(1-2):151-8. doi: 10.1016/s1383-5718(98)00060-6.
DNA damage, mainly single strand breaks, was evaluated by single cell gel electrophoresis, in leukocytes of 36 healthy and 14 thyroid cancer-affected children prior to radio-therapy. The children come from the Gomel region, one of the areas most heavily radio-contaminated by the Chernobyl fallout. In addition, leukocytes were treated with a challenge dose of bleomycin (BLM, 1.5 micrograms/ml), to assess the presence of an adaptive response (AR) potentially resulting from chronic exposure to radionuclides. As controls, 13 children living in Pisa (Italy) were enrolled in the study. Children with thyroid cancer show higher (p < 0.001) DNA damage than healthy ones. No difference was found between healthy children from Gomel and from Pisa. A reduction in the response to BLM was significantly linked to low plasma levels of FT4 hormone (p < 0.0001), to the presence of the tumor (p < 0.002), to being female (p < 0.02), and to a higher 137Cs body burden (p < 0.03).
在放疗前，通过单细胞凝胶电泳对36名健康儿童和14名患甲状腺癌儿童的白细胞中的DNA损伤（主要是单链断裂）进行了评估。这些儿童来自戈梅利地区，该地区是受切尔诺贝利辐射尘污染最严重的地区之一。此外，用博来霉素（BLM，1.5微克/毫升）激发剂量处理白细胞，以评估长期接触放射性核素可能产生的适应性反应（AR）。作为对照，招募了13名居住在意大利比萨的儿童参与该研究。患甲状腺癌的儿童比健康儿童表现出更高的（p<0.001）DNA损伤。来自戈梅利的健康儿童和来自比萨的健康儿童之间未发现差异。对BLM反应的降低与FT4激素的低血浆水平（p<0.0001）、肿瘤的存在（p<0.002）、女性性别（p<0.02）以及较高的137Cs体内负荷（p<0.03）显著相关。
