使用唾液和脑脊液通过聚合酶链反应（PCR）对人类狂犬病进行生前诊断。
Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid.
作者信息
Crepin P, Audry L, Rotivel Y, Gacoin A, Caroff C, Bourhy H
机构信息
Rabies Unit, Institut Pasteur, Paris, France.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1117-21. doi: 10.1128/JCM.36.4.1117-1121.1998.
Abstract
An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.
摘要
一种用于活体检测狂犬病病毒基因组RNA的优化逆转录（RT）-PCR方案，用从28例疑似狂犬病患者获得的临床样本进行了测试，其中9例经尸检确诊患有狂犬病。使用唾液进行RT-PCR并结合皮肤活检样本进行免疫荧光测定，可检测出这9例患者中的狂犬病病毒。
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