通过随机扩增多态性DNA分析对肠侵袭性大肠杆菌和志贺氏菌菌株进行鉴定。
Characterization of enteroinvasive Escherichia coli and Shigella strains by RAPD analysis.
作者信息
Bando S Y, do Valle G R, Martinez M B, Trabulsi L R, Moreira-Filho C A
机构信息
Departamento de Imunologia, Universidade de São Paulo, Brazil.
出版信息
FEMS Microbiol Lett. 1998 Aug 1;165(1):159-65. doi: 10.1111/j.1574-6968.1998.tb13141.x.
Genetic variation of 33 enteroinvasive Escherichia coli (EIEC), 12 non-EIEC and 39 Shigella strains (representing the 4 species of this genus) was analyzed using the random amplified polymorphic DNA (RAPD) technique. Reproducible polymorphisms were generated and the combined data allowed us to construct a dendrogram using Jaccard's distance. Two main groups were obtained: one for Shigella and the other for EIEC and non-EIEC strains. The first group contained four clusters, one for each Shigella species. The second group contained one cluster for EIEC and another for non-EIEC strains. The main clusters encompassed many small clusters corresponding to different serotypes. It was possible to characterize each one of the 84 strains under study as well as the boundaries among Shigella species and between this genus and EIEC strains.
使用随机扩增多态性DNA（RAPD）技术分析了33株侵袭性大肠杆菌（EIEC）、12株非EIEC和39株志贺氏菌菌株（代表该属的4个种）的遗传变异。产生了可重复的多态性，综合数据使我们能够使用杰卡德距离构建一个树形图。得到了两个主要组：一组是志贺氏菌，另一组是EIEC和非EIEC菌株。第一组包含四个簇，每种志贺氏菌一个簇。第二组包含一个EIEC簇和另一个非EIEC菌株簇。主要簇包含许多对应于不同血清型的小簇。有可能对所研究的84株菌株中的每一株以及志贺氏菌属物种之间以及该属与EIEC菌株之间的界限进行表征。
Zotero 插件