Warburton D W, Austin J W, Harrison B H, Sanders G
Bureau of Microbial Hazards, Food Directorate, Health Canada, Sir Frederick Banting Research Center, Ottawa, Ontario.
J Food Prot. 1998 Aug;61(8):948-52. doi: 10.4315/0362-028x-61.8.948.
A methodology used to isolate Escherichia coli O157:H7 from water and survival of this pathogen in inoculated water is described. The methodology used in the isolation of E. coli O157:H7 included the use of selective plating on Sorbitol MacConkey agar (supplemented with potassium tellurite [2.5 mg/liter], cefixime [0.05 mg/liter], and cefsulodin [10 mg/liter], and modified hemorrhagic colitis agar (also supplemented with potassium tellurite [2.5 mg/liter]) and cefsulodin [10 mg/liter]). There were no significant differences (P < 0.05) between the recoveries of E. coli O157:H7 on these two selective media. Direct plating on these selective agars was used to determine the length of time that E. coli O157:H7 was able to grow, remain viable, and be resistant to the selective agents. E. coli O157:H7 survived in inoculated water for up to > 300 days, depending on the type of water. Observation by scanning electron microscopy indicated that E. coli O157:H7 cells attached to, and multiplied on, the container walls.
本文描述了一种从水中分离大肠杆菌O157:H7的方法以及该病原体在接种水中的存活情况。分离大肠杆菌O157:H7所使用的方法包括在添加亚碲酸钾（2.5毫克/升）、头孢克肟（0.05毫克/升）和头孢磺啶（10毫克/升）的山梨醇麦康凯琼脂以及改良出血性结肠炎琼脂（同样添加亚碲酸钾[2.5毫克/升]和头孢磺啶[10毫克/升]）上进行选择性平板接种。在这两种选择性培养基上大肠杆菌O157:H7的回收率之间没有显著差异（P<0.05）。在这些选择性琼脂上直接平板接种用于确定大肠杆菌O157:H7能够生长、保持存活以及对选择剂具有抗性的时间长度。根据水的类型，大肠杆菌O157:H7在接种水中存活长达300多天。扫描电子显微镜观察表明，大肠杆菌O157:H7细胞附着在容器壁上并在其上繁殖。
