Seo Min-Goo, Ouh In-Ohk, Kwak Dongmi
College of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Bukgu, Daegu, 41566, South Korea.
Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon, Gyeongbuk, 39660, South Korea.
Trop Anim Health Prod. 2018 Aug;50(6):1399-1404. doi: 10.1007/s11250-018-1564-0. Epub 2018 Mar 16.
Coxiella burnetii is the causative agent of the zoonotic Q fever, and its reservoirs include ticks and livestock, which are key sources of transmission to humans. Although there have been several studies on the prevalence of C. burnetii antibodies in dairy cattle bulk tank milk (BTM), there is a lack of information on the molecular detection of C. burnetii in BTM in South Korea. Thus, this study was designed to assess milk shedding of C. burnetii in BTM from dairy cattle herds. Among the 607 BTM samples collected from 41 counties in Gyeongsang provinces in 2015, 108 (17.8%) from 23 (56.1%) counties tested positive for C. burnetii by PCR. Because the 16S rRNA sequences of C. burnetii from all 108 PCR-positive samples were identical, two representative samples (BTM-GB-10 and BTM-GN-63) are described in this paper. These sequences showed high identity (96.9-100%) to other C. burnetii sequences deposited in GenBank. Phylogenetic analysis showed that these two sequences were clustered with existing C. burnetii strains. The relatively high prevalence rates of C. burnetii in BTM detected in this study suggest that C. burnetii is prevalent among dairy cattle herds in South Korea. Thus, implementation of continuous monitoring and control strategies for domestic animals is needed to prevent disease transmission and protect public health.
伯氏考克斯氏体是人畜共患Q热的病原体，其宿主包括蜱虫和家畜，它们是传播给人类的主要源头。尽管已有多项关于奶牛储奶罐乳（BTM）中伯氏考克斯氏体抗体流行情况的研究，但韩国缺乏关于BTM中伯氏考克斯氏体分子检测的信息。因此，本研究旨在评估奶牛群BTM中伯氏考克斯氏体的乳汁排出情况。在2015年从庆尚南道41个县采集的607份BTM样本中，来自23个县（56.1%）的108份样本（17.8%）经PCR检测伯氏考克斯氏体呈阳性。由于所有108份PCR阳性样本的伯氏考克斯氏体16S rRNA序列相同，本文描述了两个代表性样本（BTM - GB - 10和BTM - GN - 63）。这些序列与GenBank中其他伯氏考克斯氏体序列具有高度同源性（96.9 - 100%）。系统发育分析表明，这两个序列与现有的伯氏考克斯氏体菌株聚类。本研究中检测到的BTM中伯氏考克斯氏体相对较高的流行率表明，伯氏考克斯氏体在韩国奶牛群中普遍存在。因此，需要对家畜实施持续监测和控制策略，以预防疾病传播并保护公众健康。
