Okulicz W C, Scarrell R
Department of OB/GYN, University of Massachusetts Medical School, Worcester 01655, USA.
Proc Soc Exp Biol Med. 1998 Sep;218(4):316-21. doi: 10.3181/00379727-218-44298.
During menses in the primate, zone I and parts of zone II of the endometrium are sloughed, and subsequently reconstruction of the endometrium for the next cycle begins. In this study we examined the cell type and zonal changes in estrogen (E) and progesterone (P) receptors (R) that occur during these changes in endometrial structure and the coincident changes in hormonal stimulation. Immunohistochemical analyses of ERalpha and PR were performed on endometrial biopsies obtained during artificial menstrual cycles in the rhesus monkey on day 26 (declining serum P), Days 1 and 3 of menses, and Day 5 (following endometrial reconstruction). The pattern and distribution of ERalpha and PR in the endometrium on Day 26 was similar to that observed previously on Day 23; ERalpha and PR showed strong positive staining for glandular epithelia in zone IV of the basalis and little or no staining for ERalpha in zones I and II-III whereas PR showed positive staining in stromal cells and little or no staining in epithelia in zones I and II-III. On Day 1 of menses, no detectable ERalpha and PR staining was observed in glandular epithelial throughout the endometrium. PR immunoreactivity remained in stromal cells on Day 1 whereas ERalpha staining returned to stromal cells reflecting a release from P inhibition of ERalpha. By Day 3 of menses positive staining for ERalpha and PR was observed in glandular epithelia in zones II-III and zone IV. By Day 5 the reconstruction of the endometrium was complete, and strong positive staining for both receptors was present throughout the endometrium. Following removal of all E stimulation on Day 26, no distinct differences in receptor distribution were observed on Day 3. These data suggest that hormone-independent mechanisms such as in normal wound healing are also operative during menses and reconstruction. The presence of ERalpha, primarily in stromal cells during normal menses, supports the notion that the mesenchymal compartment plays an important role in the orchestration of normal endometrial reconstruction in response to E.
在灵长类动物的月经期间，子宫内膜的I区和II区的部分会脱落，随后开始为下一个周期重建子宫内膜。在本研究中，我们检查了在子宫内膜结构的这些变化以及激素刺激的同时变化过程中，雌激素（E）和孕激素（P）受体（R）的细胞类型和区域变化。对恒河猴在人工月经周期的第26天（血清P下降）、月经第1天和第3天以及第5天（子宫内膜重建后）获得的子宫内膜活检组织进行了雌激素受体α（ERα）和孕激素受体（PR）的免疫组织化学分析。第26天子宫内膜中ERα和PR的模式和分布与之前在第23天观察到的相似；ERα和PR在基底层IV区的腺上皮中显示强阳性染色，而在I区和II - III区中ERα几乎没有或没有染色，而PR在基质细胞中显示阳性染色，在I区和II - III区的上皮中几乎没有或没有染色。在月经第1天，整个子宫内膜的腺上皮中未观察到可检测到的ERα和PR染色。PR免疫反应性在第1天仍存在于基质细胞中，而ERα染色回到基质细胞，反映出ERα从P抑制中释放出来。到月经第3天，在II - III区和IV区的腺上皮中观察到ERα和PR的阳性染色。到第5天，子宫内膜重建完成，两种受体在整个子宫内膜中均呈现强阳性染色。在第26天去除所有E刺激后，第3天未观察到受体分布的明显差异。这些数据表明，诸如正常伤口愈合中的激素非依赖性机制在月经和重建过程中也起作用。在正常月经期间，ERα主要存在于基质细胞中，这支持了间充质部分在响应E协调正常子宫内膜重建中起重要作用的观点。
