Gajadhar A A, Aramini J J, Tiffin G, Bisaillon J R
Centre for Animal Parasitology, Health of Animals Laboratory, Canadian Food and Inspection Agency, Saskatoon, Saskatchewan.
J Parasitol. 1998 Aug;84(4):759-63.
During 1991 and 1992, 2,800 market-age pigs were sampled at federally inspected abattoirs from across Canada. Anti-Toxoplasma gondii IgG at titers of > or =1:32 were found in 240 pigs examined by a commercial, latex agglutination test. Seroprevalences ranged from 3.5 to 13.2% in the different regions of the country. Tissue hybridization studies using a previously developed probe demonstrated T. gondii ribosomal RNA in 9 of 36 animals, whereas mouse bioassay testing of heart muscle and diaphragm from all 2,800 pigs failed to demonstrate the presence of infective stages of T. gondii in tissues. Although serology results from this study indicated that Canadian market-age pigs are infected with T. gondii at rates similar to those reported from other parts of North America, mouse bioassay results suggested that Canadian pork products contain low levels of infective organisms. This apparent discrepancy suggests that serological evidence of T. gondii infection in pigs alone does not accurately assess the public health risks associated with consuming improperly cooked pork products.
在1991年和1992年期间,从加拿大各地经联邦检查的屠宰场抽取了2800头适销育肥猪作为样本。通过商业乳胶凝集试验检测的240头猪中,发现抗弓形虫IgG滴度≥1:32。该国不同地区的血清阳性率在3.5%至13.2%之间。使用先前开发的探针进行的组织杂交研究在36只动物中的9只中检测到了弓形虫核糖体RNA,而对所有2800头猪的心肌和膈肌进行的小鼠生物测定试验未能证明组织中存在弓形虫感染阶段。尽管这项研究的血清学结果表明,加拿大适销育肥猪感染弓形虫的比率与北美其他地区报告的比率相似,但小鼠生物测定结果表明,加拿大猪肉产品中感染性生物体的含量较低。这种明显的差异表明,仅猪感染弓形虫的血清学证据并不能准确评估食用未煮熟猪肉产品相关的公共卫生风险。