Abraham W R, Meyer H, Yakimov M
Gesellschaft für Biotechnologische Forschung mbH, Division of Microbiology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Biochim Biophys Acta. 1998 Jul 31;1393(1):57-62. doi: 10.1016/s0005-2760(98)00058-7.
The polar lipids from the hydrocarbon using and biosurfactant-producing bacterium Alcanivorax borkumensis were isolated and identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The biosurfactant produced by this species is an anionic glucose lipid with a tetrameric oxyacyl side chain. The glycolipids extracted from the cell wall consist of this biosurfactant N-terminally esterified with glycine. Ten different derivatives of this lipid type were identified and their structures elucidated by MSMS. They vary by the chain length of one or two of the four beta-hydroxy fatty acids (C6, C8 and C10) and by the location of these different fatty acids within the molecule. All compounds are reported here for the first time. In addition to these glycolipids, three different phosphatidylglycerols were identified. While these lipids were found in all strains of A. borkumensis, the relative abundances of the different lipids vary between the strains.
利用质谱（MS）和核磁共振（NMR）光谱法对利用烃类且产生生物表面活性剂的博氏嗜油菌（Alcanivorax borkumensis）的极性脂质进行了分离和鉴定。该菌种产生的生物表面活性剂是一种具有四聚体氧酰基侧链的阴离子葡萄糖脂。从细胞壁提取的糖脂由N端用甘氨酸酯化的这种生物表面活性剂组成。鉴定出了这种脂质类型的十种不同衍生物，并通过串联质谱（MSMS）阐明了它们的结构。它们因四个β-羟基脂肪酸（C6、C8和C10）中一个或两个的链长以及这些不同脂肪酸在分子内的位置而有所不同。所有这些化合物均首次在此报道。除了这些糖脂外，还鉴定出了三种不同的磷脂酰甘油。虽然在博氏嗜油菌的所有菌株中都发现了这些脂质，但不同脂质的相对丰度在菌株之间有所不同。
