Crispin J C, Martínez A, de Pablo P, Velasquillo C, Alcocer-Varela J
Department of Immunology and Rheumatology, Instituto Nacional de la Nutrición Salvador Zubirán, Mexico City, Mexico.
Scand J Immunol. 1998 Aug;48(2):196-200. doi: 10.1046/j.1365-3083.1998.00366.x.
Accumulating evidence has implicated T cells in the pathogenesis of systemic lupus erythematosus (SLE). The CD69 antigen is an integral membrane protein rapidly induced on the surface of activated lymphocytes. We obtained CD4+ and CD8+ T cells from normal subjects and patients with SLE. The percentage of CD69 expression in freshly isolated cells and after in-vitro incubation with mitogens was quantified by three-colour immunofluorescent staining. Expression of this protein was increased in both CD4+ and CD8+ T-cell subsets from SLE patients when compared with normal cells, although the difference was significant only in the CD8+ T-cell subset (P = 0.05). Cellular activation increased CD69 expression. When stimulated with anti-CD2/CD2R or phytohaemagglutinin (PHA), the percentage and absolute numbers of CD69+ cells were lower in patients than in controls. Addition of anti-interleukin (IL)-10 monoclonal antibody (MoAb) increased the percentage of in-vitro CD69 expression in SLE cells. These results suggest that the peripheral blood lymphocytes from patients with SLE have an intrinsic defect that alters their activation process, including the expression of CD69, and might explain some of the T immunoregulatory abnormalities observed in these patients.
越来越多的证据表明，T细胞参与了系统性红斑狼疮（SLE）的发病机制。CD69抗原是一种在活化淋巴细胞表面迅速诱导产生的整合膜蛋白。我们从正常受试者和SLE患者中获取了CD4 +和CD8 + T细胞。通过三色免疫荧光染色对新鲜分离细胞以及与丝裂原体外孵育后的细胞中CD69表达的百分比进行了定量。与正常细胞相比，SLE患者的CD4 +和CD8 + T细胞亚群中该蛋白的表达均有所增加，尽管差异仅在CD8 + T细胞亚群中具有统计学意义（P = 0.05）。细胞活化会增加CD69的表达。当用抗CD2 / CD2R或植物血凝素（PHA）刺激时，患者中CD69 +细胞的百分比和绝对数量低于对照组。添加抗白细胞介素（IL）-10单克隆抗体（MoAb）可增加SLE细胞体外CD69表达的百分比。这些结果表明，SLE患者的外周血淋巴细胞存在内在缺陷，这改变了它们的活化过程，包括CD69的表达，这可能解释了在这些患者中观察到的一些T免疫调节异常。
