Asano K, Matsuishi J, Yu Y, Kasahara T, Hisamitsu T
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
Immunopharmacology. 1998 May;39(2):117-26. doi: 10.1016/s0162-3109(98)00006-x.
The effect of chloroform extract of Tripterygium wilfordii Hook f. (TWH extract), a traditional immunosuppressive Chinese herb, on type II collagen (C II)-induced arthritis (CIA) in DBA/1J mice was studied. In the first set of experiments, we examined the effect of TWH extract on cellular immune responses to C II. As compared with mice treated with saline, TWH extract administered orally at doses of more than 400 microg kg(-1) once a day for 14 days inhibited the ability of inguinal lymph node cells to produce T cell cytokines interleukin-2 and interferon-gamma when the cells were obtained from mice 21 days after immunization and cultured in vitro with C II. Treatment with TWH extract also inhibited production of macrophage cytokines interleukin-1beta and tumor necrosis factor-alpha in response to in vitro stimulation of lymph node cells with C II. In the second part of the experiment, we evaluated the influence of TWH extract on the incidence and development of arthritis in murine CIA. Mice were immunized twice at a 3-week interval with bovine C II, with TWH extract being given orally once a day for 14 days with four different regimens. A 14-day course of TWH extract treatment at a daily dose of 400 microg kg(-1), which began on the day of the first C II immunization, suppressed the development of arthritis, as well as antibody production and delayed-type hypersensitivity to C II. Treatment with TWH extract, which started on the same day as the booster immunization, also resulted in inhibition of development of arthritis and of immune responses to C II. On the other hand, therapeutic administration with TWH extract did not affect the clinical course of the disease and the immune response to C II.
雷公藤（Tripterygium wilfordii Hook f.）是一种传统的具有免疫抑制作用的中药，研究了其氯仿提取物（TWH提取物）对DBA/1J小鼠II型胶原（C II）诱导的关节炎（CIA）的影响。在第一组实验中，我们检测了TWH提取物对C II细胞免疫反应的影响。与用生理盐水处理的小鼠相比，每天口服剂量超过400μg kg⁻¹，连续14天给予TWH提取物，可抑制腹股沟淋巴结细胞产生T细胞细胞因子白细胞介素-2和干扰素-γ的能力，这些细胞是在免疫后21天从小鼠获取，并在体外与C II一起培养的。用TWH提取物处理也抑制了巨噬细胞细胞因子白细胞介素-1β和肿瘤坏死因子-α在体外被C II刺激的淋巴结细胞时的产生。在实验的第二部分，我们评估了TWH提取物对小鼠CIA关节炎发病率和发展的影响。小鼠每隔3周用牛C II免疫两次，用TWH提取物以四种不同方案每天口服一次，共14天。从第一次C II免疫当天开始，每天剂量为400μg kg⁻¹的14天TWH提取物治疗疗程，抑制了关节炎的发展以及抗体产生和对C II的迟发型超敏反应。与加强免疫同一天开始用TWH提取物治疗，也导致关节炎发展和对C II免疫反应的抑制。另一方面，用TWH提取物进行治疗性给药不影响疾病的临床进程和对C II的免疫反应。
