Blomgren H
Department of General Oncology, Karolinska Hospital, Stockholm, Sweden.
Int J Immunopharmacol. 1990;12(1):99-105. doi: 10.1016/0192-0561(90)90072-u.
RU 41.740 (Biostim) is an immunostimulating substance of bacterial origin with a reported protective activity against bacterial infections in man. The aim of the present investigation was to determine if Biostim-exposed blood mononuclear cells release soluble factors in vitro which may inhibit growth of tumor cells in culture. It was observed that exposure of mononuclear cells to Biostim at concentrations as low as 1 ng/ml for 24 h resulted in the release of factors capable of retarding growth of K562 cells. Three human glioma cell lines tested also appeared to be sensitive whereas three other cell lines were less sensitive or resistant. The growth inhibitory factors were shown to be produced by monocytes which is in line with previous findings showing that Biostim is a potent activator of monocytes-macrophages. Biostim-exposed monocytes, however, did not express any cytotoxic activity for K562 cells using a short-term 51Cr-release assay. It is concluded that Biostim may trigger monocytes to release factors with anti-tumor activity. These factors have not yet been identified and it remains to be determined if their production increases during Biostim treatment and whether this is correlated to an anti-tumor activity in man.
RU 41.740（生物刺激素）是一种源自细菌的免疫刺激物质，据报道对人类细菌感染具有保护活性。本研究的目的是确定暴露于生物刺激素的血液单核细胞是否会在体外释放可能抑制培养中肿瘤细胞生长的可溶性因子。据观察，单核细胞在低至1纳克/毫升的浓度下暴露于生物刺激素24小时，会导致释放出能够延缓K562细胞生长的因子。所测试的三种人类胶质瘤细胞系似乎也很敏感，而其他三种细胞系则不太敏感或具有抗性。已证明生长抑制因子是由单核细胞产生的，这与先前的研究结果一致，即生物刺激素是单核细胞 - 巨噬细胞的有效激活剂。然而，使用短期51铬释放试验，暴露于生物刺激素的单核细胞对K562细胞没有表现出任何细胞毒性活性。结论是，生物刺激素可能会触发单核细胞释放具有抗肿瘤活性的因子。这些因子尚未得到鉴定，其产生是否在生物刺激素治疗期间增加以及这是否与人类的抗肿瘤活性相关仍有待确定。
