Kucsera J, Pfeiffer I, Ferenczy L
Department of Microbiology, József Attila University, Szeged, Hungary.
Antonie Van Leeuwenhoek. 1998 Feb;73(2):163-8. doi: 10.1023/a:1000699626367.
Sexual activity was induced in the basidiomyceteous Phaffia rhodozyma (Xanthophyllomyces dendrorhous) by depletion of nitrogen from the culture medium. This activity involved both mating between two yeast cells and the formation of basidiospores. Mating is possibly started by a G1 phase arrest of the cell cycle, as in other yeasts. The life cycle exhibited homothallic features. Crosses between genetically marked strains, and pulse-field gel electrophoresis of the chromosomal DNA of cells derived from individual spores revealed evidence of karyogamy, meiosis and even recombination. The segregation ratio in tetrads pointed to diploid vegetative cells, which formed tetraploid zygotes and the immediate meiosis then gave rise to diploid progenies again. Apart from the type strain Phaffia rhodozyma CBS 5905, all the examined strains were able to sporulate.
通过耗尽培养基中的氮来诱导担子菌红法夫酵母（Xanthophyllomyces dendrorhous）进行有性活动。这种活动涉及两个酵母细胞之间的交配以及担孢子的形成。与其他酵母一样，交配可能是由细胞周期的G1期停滞引发的。生命周期表现出同宗配合的特征。对遗传标记菌株之间的杂交以及来自单个孢子的细胞的染色体DNA进行脉冲场凝胶电泳，揭示了核融合、减数分裂甚至重组的证据。四分体中的分离比表明存在二倍体营养细胞，其形成四倍体合子，随后立即进行减数分裂，再次产生二倍体后代。除了模式菌株红法夫酵母CBS 5905外，所有检测的菌株都能够形成孢子。
