Tziafas D, Alvanou A, Papadimitriou S, Gasic J, Komnenou A
Department of Endodontology, School of Dentistry, Greece.
Arch Oral Biol. 1998 Jun;43(6):431-44. doi: 10.1016/s0003-9969(98)00026-0.
The effects of recombinant basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF)-II and transforming growth factor (TGF)-beta 1 on dental pulp cells were investigated by light and transmission electron microscopy after their implantation for 1 and 3 weeks at central sites of mechanically exposed pulps in dog molar and canine teeth. The implants were Millipore filters that have been soaked with solutions containing 100 or 500 ng/ml of bFGF or IGF-II or 100 ng/ml of TGF-beta 1. Control filters were soaked with dog albumin. No changes in cell organization or matrix synthesis were seen after implantation of control filters. Groups of columnar, polarized cells with numerous mitochondria and Golgi elements or elongated cells unassociated with any matrix deposition were demonstrated after 1 or 3 weeks, respectively, in close proximity to the filters that had been soaked with bFGF solution; at a distance from these implants enhanced formation of an osteotypic matrix was seen beneath the exposure site. No particular response was found in close proximity to the filters that had been soaked with IGF-II solution after 1 or 3 weeks implantation but thick zones of osteodentine were found beneath the exposure site and at adjacent circumferential dentine sites. Numerous elongated, polarized cells with long cytoplasmic extensions invading the filter pores were consistently seen after 1 week in close proximity to the filters that had been soaked with TGF-beta 1 solution. After 3 weeks implantation of these filters, deposition of a tubular matrix surrounding the implants was seen in association with the highly elongated odontoblast-like cells, while enhancement of circumferential dentine formation was also found at adjacent peripheral sites. These experiments demonstrate that TGF-beta 1 when implanted for short term periods at central pulp sites exerted dentine-specific effects, inducing differentiation of odontoblast-like cells and stimulating primary odontoblasts. Implantation of bFGF and IGF-II did not result in reparative dentine formation, but did stimulate osteotypical matrix deposition at a distance from the implants.
将重组碱性成纤维细胞生长因子（bFGF）、胰岛素样生长因子（IGF）-II和转化生长因子（TGF）-β1植入犬磨牙和犬齿机械暴露牙髓的中央部位1周和3周后，通过光学显微镜和透射电子显微镜研究了它们对牙髓细胞的影响。植入物是用含有100或500 ng/ml bFGF或IGF-II或100 ng/ml TGF-β1的溶液浸泡过的密理博滤膜。对照滤膜用犬白蛋白浸泡。植入对照滤膜后，未见细胞组织或基质合成有变化。分别在植入1周或3周后，在浸泡过bFGF溶液的滤膜附近可见成群的柱状、极化细胞，有大量线粒体和高尔基体元件，或可见与任何基质沉积无关的伸长细胞；在距这些植入物一定距离处，暴露部位下方可见骨样基质形成增强。植入IGF-II溶液浸泡过的滤膜1周或3周后，在滤膜附近未发现特殊反应，但在暴露部位下方和相邻的圆周牙本质部位发现了厚层的骨样牙本质。在浸泡过TGF-β1溶液的滤膜附近，1周后始终可见许多伸长的、极化的细胞，其长细胞质突起侵入滤膜孔。植入这些滤膜3周后，可见围绕植入物沉积管状基质，伴有高度伸长的成牙本质细胞样细胞，同时在相邻外周部位也发现圆周牙本质形成增强。这些实验表明，短期植入牙髓中央部位的TGF-β1发挥了牙本质特异性作用，诱导成牙本质细胞样细胞分化并刺激初级成牙本质细胞。植入bFGF和IGF-II未导致修复性牙本质形成，但确实刺激了距植入物一定距离处的骨样基质沉积。
