Tziafas D, Alvanou A, Panagiotakopoulos N, Smith A J, Lesot H, Komnenou A, Ruch J V
Department of Dental Pathology and Therapeutics, Dental School, Aristotle University of Thessaloniki, Greece.
Arch Oral Biol. 1995 Oct;40(10):883-93. doi: 10.1016/0003-9969(95)00069-2.
The effects of dentine extracellular matrix components on dental mesenchymal cells were studied by light and transmission electron microscopy after their implantation at central sites of mechanically exposed pulps in dog molar teeth. The implants were Millipore filters that had been soaked with solutions containing 30 or 300 micrograms/ml of an EDTA-soluble fraction of rabbit incisor dentine. Control filters were soaked with dog albumin or phosphate buffered saline. Columnar, polarized cells were consistently seen after 8 days in close proximity to the filters coated with both concentrations of dentine matrix components. Characteristic features of these polarized cells included widened cisternae of the rough endoplasmic reticulum, a rich microfilamentous network in the long cytoplasmic extensions invading the filter pores and numerous cytoplasmic bodies. These cells also showed evidence of functional as well as cytological differentiation. Polarized processing of secretory granules could be observed after 8 days' implantation, and also the presence of matrix vesicles and deposition of a fine, collagenous matrix into the filters apically to the distal end of the cytoplasmic processes. After 24 days' implantation, secretion of a tubular matrix could be consistently seen in association with the odontoblast-like cells. No changes in cell organization or matrix synthesis were seen after implantation of control filters. These studies demonstrate that bioactive components present in the EDTA-soluble dentine matrix fraction are able to directly induce cell polarization and apical secretion of tubular matrix when implanted in contact with dental pulp cells at sites remote from the odontoblast layer.
将含有30或300微克/毫升兔切牙牙本质的EDTA可溶性组分的溶液浸泡过的微孔滤膜植入犬磨牙机械暴露牙髓的中央部位后，通过光学显微镜和透射电子显微镜研究牙本质细胞外基质成分对牙髓间充质细胞的影响。对照滤膜用犬白蛋白或磷酸盐缓冲盐水浸泡。在植入含有两种浓度牙本质基质成分的滤膜附近8天后，始终可见柱状、极化细胞。这些极化细胞的特征包括粗面内质网池增宽、侵入滤膜孔的长细胞质突起中有丰富的微丝网络以及大量细胞质小体。这些细胞还显示出功能和细胞学分化的证据。植入8天后可观察到分泌颗粒的极化加工，以及基质小泡的存在和在细胞质突起远端顶端向滤膜内沉积精细的胶原基质。植入24天后，与成牙本质细胞样细胞相关的管状基质分泌始终可见。植入对照滤膜后，细胞组织或基质合成未见变化。这些研究表明，当EDTA可溶性牙本质基质组分中的生物活性成分植入远离成牙本质细胞层的部位并与牙髓细胞接触时，能够直接诱导细胞极化和管状基质的顶端分泌。
