Tziafas D, Papadimitriou S
Department of Endodontology, School of Dentistry, Aristotle University of Thessaloniki, Greece.
Eur J Oral Sci. 1998 Jan;106 Suppl 1:192-6. doi: 10.1111/j.1600-0722.1998.tb02175.x.
This study was designed to investigate in vivo i) the role of TGF-beta as an active component of the dentin matrix during induction of reparative dentinogenesis; and ii) the ability of TGF-beta1 isoform to induce dentinogenic events. Dental pulps of dog molars and canines were mechanically exposed, and the following implants were placed intrapulpally for 42 d: i) Demineralized or native autogenous dentin matrix preincubated with a TGF-beta-neutralizing antibody; ii) Millipore filters soaked with solution containing 100 ng/ml of TGF-beta1 from human platelets; iii) biomatrices and filters soaked in control solutions. After incubation of biomatrices with the TGF-beta-neutralizing antibody, demineralized dentin completely lost its inductive activity, while native dentin was only able to stimulate formation of fibrodentin. Millipore filters soaked with TGF-beta1 were consistently surrounded by a thick zone of tubular matrix lined with high columnar polarized cells. The data provide evidence that pulp cells can express their dentinogenic potential in response to an appropriate surface containing exogenous TGF-beta1, and that the dentinogenic activity of dentin matrix may at least partly be ascribed to TGF-beta molecule(s).
i）转化生长因子-β（TGF-β）作为牙本质基质的活性成分在诱导修复性牙本质形成过程中的作用；ii）TGF-β1亚型诱导牙本质形成事件的能力。将犬磨牙和尖牙的牙髓进行机械暴露，并将以下植入物牙髓腔内放置42天：i）用TGF-β中和抗体预孵育的脱矿或天然自体牙本质基质；ii）用含100 ng/ml人血小板TGF-β1溶液浸泡的微孔滤膜；iii）浸泡在对照溶液中的生物基质和滤膜。用TGF-β中和抗体孵育生物基质后，脱矿牙本质完全丧失其诱导活性，而天然牙本质仅能刺激纤维牙本质的形成。用TGF-β1浸泡的微孔滤膜始终被一层由高柱状极化细胞排列的厚管状基质区域包围。数据表明，牙髓细胞可对外源TGF-β1的合适表面作出反应，表达其牙本质形成潜能，并且牙本质基质的牙本质形成活性可能至少部分归因于TGF-β分子。
