Horgen L, Sola C, Devallois A, Goh K S, Rastogi N
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Morne Jolivière, Pointe à Pitre, Guadeloupe.
FEMS Immunol Med Microbiol. 1998 Jul;21(3):203-12. doi: 10.1111/j.1574-695X.1998.tb01167.x.
A total of 115 Mycobacterium tuberculosis isolates from 80 patients were typed using IS6110-DNA fingerprinting and DR-based spoligotyping to describe the active transmission of tuberculosis in a Caribbean setting over a 2-year period. A total of 61 different pattern types were observed by IS6110-RFLP and 10 clusters containing between two and 15 patients could be defined. By spoligotyping, 45 different pattern types were observed with 12 clusters containing two to 11 patients. Thirty-two patients could be included in eight spoligotype-defined clusters and in nine RFLP-defined clusters when strictly concordant matching results were put together. In conclusion, about 40% of the patient isolates were clustered by DNA fingerprinting suggesting recent transmission of tuberculosis in our region. This study confirmed the increased accuracy and discriminatory power of the association of IS6110-RFLP and spoligotyping for studies on the molecular epidemiology of M. tuberculosis, and suggests that despite good implementation of tuberculosis control programs in Guadeloupe, active transmission of tuberculosis may be far more important than suspected.
使用IS6110 - DNA指纹图谱和基于DR的间隔寡核苷酸分型技术对来自80名患者的115株结核分枝杆菌分离株进行分型，以描述加勒比地区两年内结核病的活动性传播情况。通过IS6110 - RFLP观察到共有61种不同的模式类型，并且可以定义10个包含2至15名患者的聚类。通过间隔寡核苷酸分型，观察到45种不同的模式类型，有12个包含2至11名患者的聚类。当将严格一致的匹配结果汇总时，32名患者可被纳入8个由间隔寡核苷酸分型定义的聚类和9个由RFLP定义的聚类中。总之，约40%的患者分离株通过DNA指纹图谱聚类，表明在我们地区近期存在结核病传播。本研究证实了IS6110 - RFLP与间隔寡核苷酸分型相结合在结核分枝杆菌分子流行病学研究中准确性和鉴别能力的提高，并表明尽管瓜德罗普岛的结核病控制项目实施良好，但结核病的活动性传播可能比预期的更为重要。
