Woodhead M, Davies H V, Brennan R M, Taylor M A
Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, UK.
Mol Biotechnol. 1998 Jun;9(3):243-6. doi: 10.1007/BF02915797.
A method is described for isolating DNA of high molecular mass (M(r)) from blackcurrant and other softfruit species. Following a hexacethylytimethyl ammonium bromide (CTAB)-based extraction procedure, samples are treated with a glycosidic hydrolase mixture and RNase, and then purified. The suitability of this DNA for Southern analysis and genomic-library construction is demonstrated.
