Taylor M A, Wright F, Davies H V
Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Plant Mol Biol. 1994 Nov;26(3):1013-18. doi: 10.1007/BF00028869.
The cDNA clones of two potato beta-tubulin genes were isolated from a tuberising stolon tip library. Analysis of 20 positive clones showed that they represented one or another of two different but very similar beta-tubulin genes, designated TUBST1 and TUBST2. The expression pattern of beta-tubulin genes in the potato plant was investigated by RNA blot analysis and by RT-PCR. Southern analysis of potato genomic DNA with coding and non-coding beta-tubulin probes revealed that there are multiple beta-tubulin genes in the potato genome and that there is likely to be considerable divergence in the 3' non-coding sequences. Phylogenetic analysis of plant beta-tubulin genes is described.
从一个块茎形成的匍匐茎尖文库中分离出了两个马铃薯β-微管蛋白基因的cDNA克隆。对20个阳性克隆的分析表明，它们代表了两个不同但非常相似的β-微管蛋白基因中的一个，分别命名为TUBST1和TUBST2。通过RNA印迹分析和RT-PCR研究了β-微管蛋白基因在马铃薯植株中的表达模式。用编码和非编码β-微管蛋白探针对马铃薯基因组DNA进行Southern分析，结果表明马铃薯基因组中有多个β-微管蛋白基因，并且在3'非编码序列中可能存在相当大的差异。本文描述了植物β-微管蛋白基因的系统发育分析。
