Streilein J S, Hart D A
Infect Immun. 1976 Aug;14(2):463-70. doi: 10.1128/iai.14.2.463-470.1976.
The optimal conditions for serum-free cultures of hamster lymphoid cells were determined. The cells responded to both the nonspecific thymus-derived lymphocyte mitogen concanavalin A (Con A) and the nonspecific bone marrow-derived lymphocyte mitogens lipopolysaccharide (LPS) and dextran sulfate (DxS). Normal MHA hamster serum was shown to specifically inhibit the response to LPS and DxS by 95% without inhibiting the response to Con A. The serum also did not inhibit one-way mixed lymphocyte reaction. Incubation of cells in 5% serum for short periods of time, followed by serum-free culture, did not lead to inhibition. The serum inhibited the LPS response by 65% even when added 24 h after initiation of the culture. The activity was associated with the high-molecular-weight material on Sephadex G-200 fractionation of serum. The inhibitory fraction did not possess antibody activity to LPS. The possibility that the material is an alpha2-macroglobulin is discussed.
确定了仓鼠淋巴细胞无血清培养的最佳条件。这些细胞对非特异性胸腺来源的淋巴细胞促有丝分裂原刀豆球蛋白A（Con A）以及非特异性骨髓来源的淋巴细胞促有丝分裂原脂多糖（LPS）和硫酸葡聚糖（DxS）均有反应。已证明正常MHA仓鼠血清可特异性抑制对LPS和DxS的反应达95%，而不抑制对Con A的反应。该血清也不抑制单向混合淋巴细胞反应。细胞在5%血清中短期孵育后再进行无血清培养，不会导致抑制。即使在培养开始后24小时添加该血清，它仍能抑制65%的LPS反应。该活性与血清经Sephadex G - 200分级分离后的高分子量物质有关。抑制组分对LPS不具有抗体活性。讨论了该物质可能是α2 - 巨球蛋白的可能性。
