Prakash C, Coutinho A, Möller G
Scand J Immunol. 1976;5(1-2):77-85. doi: 10.1111/j.1365-3083.1976.tb02994.x.
We studied the inhibitory effect of a fraction of bovine seminal plasma on in vitro lymphocyte responses to specific and nonspecific ligands. Seminal plasma was fractionated by Sephadex G-100 chromatography, and the inhibitory component was found to have a molecular weight of above 100,000 daltons. This fraction was devoid of lymphocytotoxicity and could inhibit, in a dose-dependent mode, both the primary antibody response of normal mouse spleen cells to the thymus-dependent antigen sheep erythrocytes and the one-way mixed lymphocyte reactions. Concanavalin A-induced proliferative T-cell responses were also inhibited by the same fraction. The inhibition of the T-lymphocyte responses to Con A was complete at suboptimal concentrations of the mitogen and could only partially be overcome at supraoptimal concentrations. Four other fractions in seminal plasma were also studied in this particular system, three being without effect and one being stimulatory. The inhibitory fraction was also found to inhibit the polyclonal B-cell responses induced by fetal calf serum. The importance of the present findings for the immunophysiology of reproduction are discussed.
我们研究了牛精浆的一个组分对体外淋巴细胞对特异性和非特异性配体反应的抑制作用。通过葡聚糖G-100柱色谱法对精浆进行分级分离，发现抑制成分的分子量高于100,000道尔顿。该组分无淋巴细胞毒性，且能以剂量依赖方式抑制正常小鼠脾细胞对胸腺依赖性抗原绵羊红细胞的初次抗体反应以及单向混合淋巴细胞反应。刀豆球蛋白A诱导的增殖性T细胞反应也受到该组分的抑制。在次优浓度的丝裂原作用下，对Con A的T淋巴细胞反应抑制完全，而在超优浓度下只能部分被克服。在这个特定系统中还研究了精浆中的其他四个组分，其中三个无作用，一个具有刺激作用。还发现抑制性组分能抑制胎牛血清诱导的多克隆B细胞反应。讨论了本研究结果对生殖免疫生理学的重要性。
