Zaghawa A
Department of Animal and Forensic Medicine, Faculty of Veterinary Medicine, Alexandria University, Egypt.
Zentralbl Veterinarmed B. 1998 Aug;45(6):345-51. doi: 10.1111/j.1439-0450.1998.tb00803.x.
A total of 62 sera collected from cattle, buffalo, sheep, goats and camels were investigated for the presence of antibodies to bovine viral diarrhoea (BVD) virus. The prevalence of neutralizing antibodies to BVD virus was 49.2, 52.0, 27.5, 31.4 and 52.5% in cattle, buffalo, sheep, goats and camels, respectively. The positive sera were titrated against BVD virus (BVDV) strains NADL and Oregon C24V; the latter is closely related to border disease (BD) virus. The frequency distributions of the antibody titres to both strains are presented. The statistical analysis shows no significant difference between the antibody titres to BVDV strains NADL and Oregon C24V in cattle, buffalo, sheep, goats and camels. On the other hand antibody titres to BVDV were significantly higher (P < 0.05) in cattle and buffalo in comparison with sheep, goats and camels. The cell-bound immunoassay (CBIA) is a serological rest established for the detection and titration of antibodies to BVD virus and BD virus. The percentage of agreement between the CBIA and the neutralizing peroxidase-linked antibody (NPLA) test was 96.1 and 95.3% in cattle and buffalo, respectively. The sensitivity of the CBIA in comparison with the NPLA was 100% while the specificity was 92.3 and 90.3% when testing the sera of cattle and buffalo, respectively. The method is easy to perform, cheap and suitable for the conditions in Egypt.
对从牛、水牛、绵羊、山羊和骆驼采集的62份血清进行了检测，以确定是否存在针对牛病毒性腹泻（BVD）病毒的抗体。牛、水牛、绵羊、山羊和骆驼中针对BVD病毒的中和抗体流行率分别为49.2%、52.0%、27.5%、31.4%和52.5%。用BVD病毒（BVDV）毒株NADL和俄勒冈C24V对阳性血清进行滴定；后者与边界病（BD）病毒密切相关。给出了针对这两种毒株的抗体滴度的频率分布。统计分析表明，牛、水牛、绵羊、山羊和骆驼中针对BVDV毒株NADL和俄勒冈C24V的抗体滴度没有显著差异。另一方面，与绵羊、山羊和骆驼相比，牛和水牛中针对BVDV的抗体滴度显著更高（P < 0.05）。细胞结合免疫测定（CBIA）是一种用于检测和滴定针对BVD病毒和BD病毒抗体的血清学检测方法。在牛和水牛中，CBIA与中和过氧化物酶联抗体（NPLA）试验之间的一致性百分比分别为96.1%和95.3%。与NPLA相比，CBIA检测牛血清时的灵敏度为100%，特异性分别为92.3%和90.3%，检测水牛血清时也是如此。该方法操作简便、成本低廉，适合埃及的条件。
