Braun U, Bachofen C, Schenk B, Hässig M, Peterhans E
Department of Farm Animals, University of Zurich.
Schweiz Arch Tierheilkd. 2013 May;155(5):293-8. doi: 10.1024/0036-7281/a000460.
The purpose of this study was to examine the occurrence of sheep persistently infected with Border disease virus (BDV) on 76 mixed cattle and sheep farms and whether seroconversion to BDV infection occurred in cattle of these farms. Seroprevalence of BDV and bovine viral disease virus (BVDV) infection in sheep was also investigated. Quantitative RT-PCR for pestivirus detection and an ELISA to detect pestivirus antibodies were used in 2'384 and 2'291 ovine blood samples, respectively. Another 27 seropositive sheep from ten flocks underwent serum neutralization testing to differentiate between BDV and BVDV antibodies. A BDV titre that was at least four times higher than the BVDV titre was interpreted as the result of BDV infection. Titres against BVDV were interpreted in an analogous fashion. All examined sheep were pestivirus-negative, 310 sheep were seropositive, 119 had an indeterminate titre and 1'862 were seronegative. The flock seroprevalence ranged from 0.0 to 73.9 %. Three of the 27 flocks that underwent serum neutralization testing were interpreted as BDV-infected because of 6 sheep with higher BDV titres, and 6 flocks were interpreted as BVDV-infected because of 14 sheep with higher BVDV titres.
本研究的目的是调查76个牛羊混合养殖场中持续感染边界病病毒（BDV）的绵羊的发生情况，以及这些养殖场的牛是否发生了BDV感染的血清转化。还调查了绵羊中BDV和牛病毒性疾病病毒（BVDV）感染的血清流行率。分别对2384份和2291份绵羊血样进行了用于检测瘟病毒的定量RT-PCR和用于检测瘟病毒抗体的ELISA。来自十个羊群的另外27只血清阳性绵羊接受了血清中和试验，以区分BDV和BVDV抗体。BDV滴度至少比BVDV滴度高四倍被解释为BDV感染的结果。针对BVDV的滴度以类似方式解释。所有检测的绵羊瘟病毒均为阴性，310只绵羊血清阳性，119只滴度不确定，1862只血清阴性。羊群血清流行率在0.0%至73.9%之间。接受血清中和试验的27个羊群中，有3个因6只绵羊BDV滴度较高而被解释为感染了BDV，6个羊群因14只绵羊BVDV滴度较高而被解释为感染了BVDV。
