Werner P, Raducha M G, Prociuk U, Lyons L A, Kehler J S, Henthorn P S, Patterson D F
Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6010, USA.
Anim Genet. 1998 Jun;29(3):220-3. doi: 10.1046/j.1365-2052.1998.00328.x.
Previous results showed that loci from human chromosome 17q (HSA17q) map to the centromeric two-thirds of dog chromosome 9 (CFA9). In these studies fluorescence in situ hybridization (FISH) using a human total chromosome 17 painting probe, indicated that the telomeric one-third of CFA9 must have homology to one or more human chromosomes other than HSA17. Here we report that this distal part of CFA9 contains a segment syntenic to the telomeric end of HSA9q and mouse chromosome 2 (MMU2). The gene loci encoding retinoid X receptor, alpha (RXRA) and heat shock protein 5 (HSPA5 or GRP78), which are found on HSA9q34 and MMU2, occupy a region on CFA9 distal to NF1 and CRYBA1. FISH of a canine specific genomic cosmid clone for RXRA demonstrated the more telomeric localization of this locus to NF1 on CFA9. A linkage map developed for the distal region of CFA9 included: NF1-(2.7 CM)-CRYBA1-(6.5 CM)-RXRA-(22CM)-HSPA5. The next best order, RXRA-NF1-CRYBA1-HSPA5 with a difference in the log odds of 1.43 does not correspond to our findings with FISH. The most probable map order places HSPA5 distal to RXRA on CFA9 whereas in humans it lies centromeric of RXRA on HSA9q34.
先前的研究结果表明，人类染色体17q（HSA17q）上的基因座映射到犬染色体9（CFA9）着丝粒的三分之二区域。在这些研究中，使用人类17号全染色体涂染探针进行荧光原位杂交（FISH）显示，CFA9的端粒三分之一区域必定与HSA17以外的一条或多条人类染色体具有同源性。在此我们报告，CFA9的这一远端部分包含一个与HSA9q端粒末端和小鼠染色体2（MMU2）同线的片段。位于HSA9q34和MMU2上的编码视黄酸X受体α（RXRA）和热休克蛋白5（HSPA5或GRP78）的基因座，在CFA9上位于神经纤维瘤蛋白1（NF1）和β-晶体蛋白A1（CRYBA1）远端的一个区域。针对RXRA的犬特异性基因组粘粒克隆进行的FISH显示，该基因座在CFA9上相对于NF1更靠近端粒定位。为CFA9远端区域构建的连锁图谱包括：NF1-（2.7厘摩）-CRYBA1-（6.5厘摩）-RXRA-（22厘摩）-HSPA5。次优顺序RXRA-NF1-CRYBA1-HSPA5的对数优势差异为1.43，与我们FISH的结果不符。最可能的图谱顺序是HSPA5在CFA9上位于RXRA远端，而在人类中它位于HSA9q34上RXRA的着丝粒侧。
