Quesada J M, Alonso J, Gonzalez J, Muñoz R, Jans I, Martiu A, Bouillon R
Unidad de Metabolismo Mineral, Hospital Universitario Reina Sofia, Facultad de Medicina y Departamento de Biología Celular, Universidad de Córdoba, Spain.
Mech Ageing Dev. 1998 May 15;102(2-3):293-8. doi: 10.1016/s0047-6374(98)00019-0.
Beta-2 microglobulin (beta2m), the water soluble extrinsic light chain of class I MHC, has been recently isolated from the adult bone culture medium. Serum beta2m plays a role as a bone-derived growth factor regulating both osteoblast and osteoclast cell activity. Serum beta2m has been proposed as a bone remodeling biological marker in high bone turnover conditions. The purpose of our study was to determine the relationship between beta2m and vitamin D status in post-menopausal women. We have studied 44 healthy women from 20 to 80 years with normal hepatic and renal function, without diabetes mellitus and/or inflammatory, tumoral or infectious diseases. We measured the serum levels of calcium, phosphorus, parathyroid hormone (PTH), vitamin D binding protein (DBP), 25-OHD3 (calcidiol), 1,25(OH)2D3 (calcitriol) and beta2m. Serum beta2m levels increased with age (r = 0.54, P < 0.001). Post-menopausal women had higher serum levels than pre-menopausal women of beta2m (1.76 +/- 0.22 mg/l vs. 1.35 +/- 0.2 mg/l, P < 0.01); PTH (61.5 +/- 7.5 ng/ml vs. 39 +/- 6 ng/ml, P < 0.001) and lower serum levels of 25-OHD3 (7.5 +/- 2.3 ng/ml vs. 18.2 +/- 2.5 ng/ml, P < 0.001). Moreover, serum levels of beta2m were negatively correlated with 25-OHD3 (r = -0.34, P < 0.05) and with ionized calcium (r = -0.45, P < 0.01) and positively with PTH (r = 0.48, P < 0.01). These results support the role of beta2m as a regulator of bone metabolism and its potential use as a marker of high bone turnover in post-menopausal women, specially in elderly women with vitamin D deficiency and secondary hyperparathyroidism.
β2微球蛋白（β2m）是I类主要组织相容性复合体的水溶性外部轻链，最近已从成人骨培养基中分离出来。血清β2m作为一种骨源性生长因子，对成骨细胞和破骨细胞的活性均有调节作用。血清β2m已被提议作为高骨转换状态下骨重塑的生物学标志物。我们研究的目的是确定绝经后女性中β2m与维生素D状态之间的关系。我们研究了44名年龄在20至80岁之间、肝肾功能正常、无糖尿病和/或炎症、肿瘤或感染性疾病的健康女性。我们测量了血清钙、磷、甲状旁腺激素（PTH）、维生素D结合蛋白（DBP）、25-羟基维生素D3（骨化二醇）、1,25-二羟基维生素D3（骨化三醇）和β2m的水平。血清β2m水平随年龄增长而升高（r = 0.54，P < 0.001）。绝经后女性的血清β2m水平高于绝经前女性（1.76±0.22mg/l对1.35±0.2mg/l，P < 0.01）；PTH（61.5±7.5ng/ml对39±6ng/ml，P < 0.001），而血清25-羟基维生素D3水平较低（7.5±2.3ng/ml对18.2±2.5ng/ml，P < 0.001）。此外，血清β2m水平与25-羟基维生素D3呈负相关（r = -0.34，P < 0.05），与离子钙呈负相关（r = -0.45，P < 0.01），与PTH呈正相关（r = 0.48，P < 0.01）。这些结果支持了β2m作为骨代谢调节剂的作用及其作为绝经后女性高骨转换标志物的潜在用途，特别是在维生素D缺乏和继发性甲状旁腺功能亢进的老年女性中。
