• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Domain organization and functional analysis of Thermus thermophilus MutS protein.嗜热栖热菌MutS蛋白的结构域组织与功能分析
Nucleic Acids Res. 1998 Sep 15;26(18):4153-9. doi: 10.1093/nar/26.18.4153.
2
Thermus thermophilus MutS2, a MutS paralogue, possesses an endonuclease activity promoted by MutL.嗜热栖热菌MutS2是一种MutS旁系同源物，具有由MutL促进的核酸内切酶活性。
J Biochem. 2004 Mar;135(3):375-84. doi: 10.1093/jb/mvh045.
3
Mismatch DNA recognition protein from an extremely thermophilic bacterium, Thermus thermophilus HB8.来自嗜热栖热菌HB8的错配DNA识别蛋白。
Nucleic Acids Res. 1996 Feb 15;24(4):640-7. doi: 10.1093/nar/24.4.640.
4
DNA binding and protein-protein interaction sites in MutS, a mismatched DNA recognition protein from Thermus thermophilus HB8.嗜热栖热菌HB8的错配DNA识别蛋白MutS中的DNA结合位点和蛋白质-蛋白质相互作用位点。
J Biol Chem. 2000 Dec 29;275(52):40703-9. doi: 10.1074/jbc.M007124200.
5
Construction and purification of his6-Thermus thermophilus MutS protein.His6-嗜热栖热菌MutS蛋白的构建与纯化
Protein Expr Purif. 2003 Mar;28(1):69-77. doi: 10.1016/s1046-5928(02)00649-6.
6
Heteroduplex DNA and ATP induced conformational changes of a MutS mismatch repair protein from Thermus aquaticus.异源双链DNA和ATP诱导嗜热水生栖热菌MutS错配修复蛋白的构象变化。
Biochem J. 2000 May 1;347 Pt 3(Pt 3):881-6.
7
Direct observation of three conformations of MutS protein regulated by adenine nucleotides.直接观察由腺嘌呤核苷酸调节的MutS蛋白的三种构象。
J Mol Biol. 2001 May 25;309(1):227-38. doi: 10.1006/jmbi.2001.4752.
8
Domain structure of Thermus thermophilus UvrB protein. Similarity in domain structure to a helicase.嗜热栖热菌UvrB蛋白的结构域结构。与解旋酶的结构域结构相似性。
J Biol Chem. 1997 Sep 5;272(36):22703-13. doi: 10.1074/jbc.272.36.22703.
9
Identification and characterization of a thermostable MutS homolog from Thermus aquaticus.嗜热水生栖热菌中一种热稳定MutS同源物的鉴定与表征
J Biol Chem. 1996 Mar 1;271(9):5040-8. doi: 10.1074/jbc.271.9.5040.
10
Analysis of the mismatch and insertion/deletion binding properties of Thermus thermophilus, HB8, MutS.嗜热栖热菌HB8 MutS的错配及插入/缺失结合特性分析
Biochem Biophys Res Commun. 1997 Apr 28;233(3):834-7. doi: 10.1006/bbrc.1997.6551.

引用本文的文献

1
Reducing MSH4 copy number prevents meiotic crossovers between non-homologous chromosomes in Brassica napus.降低 MSH4 拷贝数可防止油菜中非同源染色体间的减数分裂交叉。
Nat Commun. 2019 May 29;10(1):2354. doi: 10.1038/s41467-019-10010-9.
2
DNA mismatch repair in eukaryotes and bacteria.真核生物和细菌中的DNA错配修复
J Nucleic Acids. 2010 Jul 27;2010:260512. doi: 10.4061/2010/260512.
3
A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways.一种新型的单链DNA特异性3'-5'核酸外切酶，嗜热栖热菌核酸外切酶I，参与多种DNA修复途径。
Nucleic Acids Res. 2010 Sep;38(17):5692-705. doi: 10.1093/nar/gkq350. Epub 2010 May 10.
4
The origins and early evolution of DNA mismatch repair genes--multiple horizontal gene transfers and co-evolution.DNA错配修复基因的起源与早期进化——多次水平基因转移与共同进化
Nucleic Acids Res. 2007;35(22):7591-603. doi: 10.1093/nar/gkm921. Epub 2007 Oct 26.
5
Nuclease activity of the MutS homologue MutS2 from Thermus thermophilus is confined to the Smr domain.嗜热栖热菌的MutS同源物MutS2的核酸酶活性局限于Smr结构域。
Nucleic Acids Res. 2007;35(3):850-60. doi: 10.1093/nar/gkl735. Epub 2007 Jan 10.
6
Substoichiometric shifting in the plant mitochondrial genome is influenced by a gene homologous to MutS.植物线粒体基因组中的亚化学计量移位受一个与MutS同源的基因影响。
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5968-73. doi: 10.1073/pnas.1037651100. Epub 2003 May 1.
7
Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches.错配结合蛋白MutS对含有不同错配的异源双链体的亲和力。
Biochem J. 2001 Mar 15;354(Pt 3):627-33. doi: 10.1042/0264-6021:3540627.
8
Genetic analysis of an incomplete mutS gene from Pseudomonas putida.恶臭假单胞菌中一个不完整mutS基因的遗传分析。
J Bacteriol. 2000 Sep;182(18):5278-9. doi: 10.1128/JB.182.18.5278-5279.2000.
9
Heteroduplex DNA and ATP induced conformational changes of a MutS mismatch repair protein from Thermus aquaticus.异源双链DNA和ATP诱导嗜热水生栖热菌MutS错配修复蛋白的构象变化。
Biochem J. 2000 May 1;347 Pt 3(Pt 3):881-6.

嗜热栖热菌MutS蛋白的结构域组织与功能分析

Domain organization and functional analysis of Thermus thermophilus MutS protein.

作者信息

Tachiki H, Kato R, Masui R, Hasegawa K, Itakura H, Fukuyama K, Kuramitsu S

机构信息

Department of Biology, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.

出版信息

Nucleic Acids Res. 1998 Sep 15;26(18):4153-9. doi: 10.1093/nar/26.18.4153.

DOI:10.1093/nar/26.18.4153
PMID:9722634
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147827/
Abstract

MutS protein binds to DNA and specifically recognizes mismatched or small looped out heteroduplex DNA. In order to elucidate its structure-function relationships, the domain structure of Thermus thermophilus MutS protein was studied by performing denaturation experiments and limited proteolysis. The former suggested that T. thermophilus MutS consists of at least three domains with estimated stabilities of 12.3, 22.9 and 30.7 kcal/mol and the latter revealed that it consists of four domains: A1 (N-terminus to residue 130), A2 (131-274), B (275-570) and C (571 to C-terminus). A gel retardation assay indicated that T.thermophilus MutS interacts non-specifically with double-stranded (ds), but not single-stranded DNA. Among the proteolytic fragments, the B domain bound to dsDNA. On the basis of these results we have proposed the domain organization of T. thermophilus MutS and putative roles of these domains.

摘要

MutS蛋白与DNA结合，并特异性识别错配或小的环状异源双链DNA。为了阐明其结构-功能关系，通过进行变性实验和有限蛋白酶解研究了嗜热栖热菌MutS蛋白的结构域结构。前者表明嗜热栖热菌MutS至少由三个结构域组成，估计稳定性分别为12.3、22.9和30.7千卡/摩尔，后者表明它由四个结构域组成：A1（N端至第130位残基）、A2（131-274）、B（275-570）和C（571至C端）。凝胶阻滞试验表明，嗜热栖热菌MutS与双链（ds）而非单链DNA非特异性相互作用。在蛋白水解片段中，B结构域与dsDNA结合。基于这些结果，我们提出了嗜热栖热菌MutS的结构域组织以及这些结构域的假定作用。