Numabe Y, Ogawa T, Kamoi H, Kiyonobu K, Sato S, Kamoi K, Deguchi S
Department of Periodontology, School of Dentistry at Tokyo, Nippon Dental University,
Ann Periodontol. 1998 Jul;3(1):102-7. doi: 10.1902/annals.1998.3.1.102.
Alterations in polymorphonuclear leukocyte (PMN) functions, such as phagocytosis, chemotaxis, and oxidative burst, play a pivotal role in periodontal pathogenesis. In addition, previous studies have demonstrated a strong relationship between smoking and periodontal disease. In the present study, the effect of cigarette smoking or passive smoking (secondary smoking) on the phagocytic function of salivary PMN (SPMN) was investigated. Twenty volunteers with clinically healthy gingiva (10 smokers, 10 non-smokers) participated in this study. In a small room, the smokers and passive smokers (non-smokers) were instructed to smoke and breathe, respectively, in an identical, specific way for about 4 minutes. SPMN was isolated immediately before and after smoking or passive smoking. PMN was then incubated with fluoresbrite beads for 45 minutes at 37 degrees C and the phagocytic status estimated by using a flow cytometer. Cell viability was determined by trypan blue exclusion (smokers before smoking: 88.3%: smokers after smoking: 89.6%: non-smokers before passive smoking: 89.0%; non-smokers after passive smoking: 89.4%). In both smokers and passive smokers, the proportion of phagocytic cells increased between before and after smoking (smokers before: 33.2%; after: 42.1%: passive smokers before: 36.2%: after: 44.1%). Both increases were statistically significant (P < 0.01). These results demonstrate that the phagocytic activity of SPMN intensifies after smoking and passive smoking. They also suggest that certain substances in cigarette smoke, perhaps nicotine, overstimulate the host response in the oral cavity.
多形核白细胞（PMN）功能的改变，如吞噬作用、趋化性和氧化爆发，在牙周病发病机制中起关键作用。此外，先前的研究表明吸烟与牙周病之间存在密切关系。在本研究中，调查了吸烟或被动吸烟（二手烟）对唾液PMN（SPMN）吞噬功能的影响。20名临床牙龈健康的志愿者（10名吸烟者，10名非吸烟者）参与了本研究。在一个小房间里，分别指导吸烟者和被动吸烟者（非吸烟者）以相同的特定方式吸烟和呼吸约4分钟。在吸烟或被动吸烟前后立即分离SPMN。然后将PMN与荧光微球在37℃下孵育45分钟，并使用流式细胞仪评估吞噬状态。通过台盼蓝拒染法测定细胞活力（吸烟者吸烟前：88.3%；吸烟者吸烟后：89.6%；非吸烟者被动吸烟前：89.0%；非吸烟者被动吸烟后：89.4%）。吸烟者和被动吸烟者在吸烟前后吞噬细胞的比例均增加（吸烟者吸烟前：33.2%；吸烟后：42.1%；被动吸烟者吸烟前：36.2%；吸烟后：44.1%）。这两种增加均具有统计学意义（P<0.01）。这些结果表明，吸烟和被动吸烟后SPMN的吞噬活性增强。它们还表明，香烟烟雾中的某些物质，可能是尼古丁，过度刺激了口腔中的宿主反应。
