Tan H, Le D, Li J, Wei W, Yao S
New Material Research Institute, Chemistry and Chemical Engineering College, Hunan University, Changsha, China.
Lett Appl Microbiol. 1998 Jul;27(1):57-61. doi: 10.1046/j.1472-765x.1998.00385.x.
A novel bulk acoustic wave (BAW) bacterial growth biosensor was developed to study in vitro susceptibility by continuous monitoring of disturbances of bacterial growth at low antibiotic concentrations, followed by the accurate and rapid estimation of growth kinetic parameters and minimum inhibitory concentrations (MICs). The susceptibilities of bacteria, e.g. Escherichia coli, Staphylococcus aureus, Proteus vulgaris, Pr. morganii and Pr. mirabilis, to various antibiotics, e.g. penicillin, streptomycin, gentamicin and cefotaxime, were investigated, respectively, and the MICs were rapidly determined with a higher reproducibility than the conventional broth micro-dilution technique (BMDT). The effects of cell constant of conductivity electrode, pH and temperature on bacterial growth and biosensor signals were discussed in detail. The proposed method offers an effective alternative to the conventional methods.
开发了一种新型体声波（BAW）细菌生长生物传感器，通过连续监测低抗生素浓度下细菌生长的干扰情况，随后准确快速地估计生长动力学参数和最低抑菌浓度（MIC），以研究体外药敏性。分别研究了大肠杆菌、金黄色葡萄球菌、普通变形杆菌、摩根氏变形杆菌和奇异变形杆菌等细菌对青霉素、链霉素、庆大霉素和头孢噻肟等各种抗生素的药敏性，并与传统肉汤微量稀释技术（BMDT）相比，以更高的重现性快速测定了MIC。详细讨论了导电电极的细胞常数、pH值和温度对细菌生长和生物传感器信号的影响。所提出的方法为传统方法提供了一种有效的替代方案。
