Liu Jonathan T C, Mandella Michael J, Friedland Shai, Soetikno Roy, Crawford James M, Contag Christopher H, Kino Gordon S, Wang Thomas D
Ginzton Laboratory, Stanford University, Stanford, California 94305, USA.
J Biomed Opt. 2006 Sep-Oct;11(5):054019. doi: 10.1117/1.2363363.
A dual-axes confocal reflectance microscope has been developed that utilizes a narrowband laser at 1310 nm to achieve high axial resolution, image contrast, field of view, and tissue penetration for distinguishing among normal, hyperplastic, and dysplastic colonic mucosa ex vivo. Light is collected off-axis using a low numerical aperture objective to obtain vertical image sections, with 4- to 5-microm resolution, at tissue depths up to 610 microm. Post-objective scanning enables a large field of view (610 x 640 microm), and balanced-heterodyne detection provides sensitivity to collect vertical sections at one frame per second. System optics are optimized to effectively reject out-of-focus scattered light without use of a low-coherence gate. This design is scalable to millimeter dimensions, and the results demonstrate the potential for a miniature instrument to detect precancerous tissues, and hence to perform in vivo histopathology.
已开发出一种双轴共聚焦反射显微镜，它利用1310纳米的窄带激光来实现高轴向分辨率、图像对比度、视野和组织穿透能力，以便在体外区分正常、增生和发育异常的结肠黏膜。使用低数值孔径物镜离轴收集光，以在高达610微米的组织深度处获得垂直图像切片，分辨率为4至5微米。物镜后扫描可实现大视野（610×640微米），平衡外差检测提供每秒一帧收集垂直切片的灵敏度。系统光学器件经过优化，无需使用低相干门就能有效抑制离焦散射光。这种设计可扩展到毫米尺寸，结果表明微型仪器有检测癌前组织的潜力，从而可用于进行体内组织病理学检查。
