用于鉴别结肠肿瘤的双轴共焦反射显微镜。
Dual-axes confocal reflectance microscope for distinguishing colonic neoplasia.
作者信息
Liu Jonathan T C, Mandella Michael J, Friedland Shai, Soetikno Roy, Crawford James M, Contag Christopher H, Kino Gordon S, Wang Thomas D
机构信息
Ginzton Laboratory, Stanford University, Stanford, California 94305, USA.
出版信息
J Biomed Opt. 2006 Sep-Oct;11(5):054019. doi: 10.1117/1.2363363.
Abstract
A dual-axes confocal reflectance microscope has been developed that utilizes a narrowband laser at 1310 nm to achieve high axial resolution, image contrast, field of view, and tissue penetration for distinguishing among normal, hyperplastic, and dysplastic colonic mucosa ex vivo. Light is collected off-axis using a low numerical aperture objective to obtain vertical image sections, with 4- to 5-microm resolution, at tissue depths up to 610 microm. Post-objective scanning enables a large field of view (610 x 640 microm), and balanced-heterodyne detection provides sensitivity to collect vertical sections at one frame per second. System optics are optimized to effectively reject out-of-focus scattered light without use of a low-coherence gate. This design is scalable to millimeter dimensions, and the results demonstrate the potential for a miniature instrument to detect precancerous tissues, and hence to perform in vivo histopathology.
摘要
已开发出一种双轴共聚焦反射显微镜，它利用1310纳米的窄带激光来实现高轴向分辨率、图像对比度、视野和组织穿透能力，以便在体外区分正常、增生和发育异常的结肠黏膜。使用低数值孔径物镜离轴收集光，以在高达610微米的组织深度处获得垂直图像切片，分辨率为4至5微米。物镜后扫描可实现大视野（610×640微米），平衡外差检测提供每秒一帧收集垂直切片的灵敏度。系统光学器件经过优化，无需使用低相干门就能有效抑制离焦散射光。这种设计可扩展到毫米尺寸，结果表明微型仪器有检测癌前组织的潜力，从而可用于进行体内组织病理学检查。
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本文引用的文献
1
Optical heterodyne scanning microscope.光学外差扫描显微镜。
Appl Opt. 1973 Nov 1;12(11):2768-72. doi: 10.1364/AO.12.002768.
2
Micromachined scanning confocal optical microscope.微机械扫描共焦光学显微镜
Opt Lett. 1996 May 15;21(10):764-6. doi: 10.1364/ol.21.000764.
3
Optical coherence microscopy in scattering media.散射介质中的光学相干显微镜术。
Opt Lett. 1994 Apr 15;19(8):590-2. doi: 10.1364/ol.19.000590.
4
Slit-scanning confocal microendoscope for high-resolution in vivo imaging.用于高分辨率体内成像的 slit 扫描共聚焦微型内窥镜。
Appl Opt. 1999 Dec 1;38(34):7133-44. doi: 10.1364/ao.38.007133.
5
Confocal microscope with large field and working distance.具有大视野和工作距离的共聚焦显微镜。
Appl Opt. 1999 Aug 1;38(22):4870-5. doi: 10.1364/ao.38.004870.
6
Real-time in vivo imaging of human gastrointestinal ultrastructure by use of endoscopic optical coherence tomography with a novel efficient interferometer design.使用具有新型高效干涉仪设计的内镜光学相干断层扫描技术对人体胃肠道超微结构进行实时体内成像。
Opt Lett. 1999 Oct 1;24(19):1358-60. doi: 10.1364/ol.24.001358.
7
Ultrahigh-resolution and 3-dimensional optical coherence tomography ex vivo imaging of the large and small intestines.超高分辨率三维光学相干断层扫描对大肠和小肠的离体成像
Gastrointest Endosc. 2005 Oct;62(4):561-74. doi: 10.1016/j.gie.2005.05.006.
8
Fibered confocal fluorescence microscopy (Cell-viZio) facilitates extended imaging in the field of microcirculation. A comparison with intravital microscopy.纤维共聚焦荧光显微镜（Cell-viZio）有助于在微循环领域进行扩展成像。与活体显微镜的比较。
J Vasc Res. 2004 Sep-Oct;41(5):400-11. doi: 10.1159/000081209. Epub 2004 Sep 30.
9
Confocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo.共聚焦激光内镜在体内诊断上皮内瘤变和结直肠癌。
Gastroenterology. 2004 Sep;127(3):706-13. doi: 10.1053/j.gastro.2004.06.050.
10
Detection of invasive colon cancer using a novel, targeted, library-derived fluorescent peptide.使用一种新型的、靶向的、源自文库的荧光肽检测浸润性结肠癌。
Cancer Res. 2004 Sep 1;64(17):6247-51. doi: 10.1158/0008-5472.CAN-04-0817.
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