Ganal M W, Czihal R, Hannappel U, Kloos D U, Polley A, Ling H Q
Institute for Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany.
Genome Res. 1998 Aug;8(8):842-7. doi: 10.1101/gr.8.8.842.
The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comparative mapping of genes across distantly related plant species by direct comparison of DNA sequences and map positions. [cDNA sequence data reported in this paper have been submitted to the EMBL database under accession nos. AA824695-AA825005 and the dbEST_Id database under accession nos. 1546519-1546862.]
番茄（Lycopersicon esculentum）的高密度限制性片段长度多态性（RFLP）连锁图谱包含300多个匿名cDNA克隆。在这些克隆中，272个已进行了部分或全序列测定。这些序列在DNA和蛋白质水平上与数据库中的已知基因进行了比较。对于57%的克隆，发现与先前描述的基因有显著匹配。这些信息将使那些标记能够转化为序列标签位点（STS）标记，并允许它们用于基于聚合酶链反应（PCR）的作图实验。此外，通过直接比较DNA序列和图谱位置，将有助于对远缘植物物种的基因进行比较作图。[本文报道的cDNA序列数据已提交至EMBL数据库，登录号为AA824695 - AA825005，以及dbEST_Id数据库，登录号为1546519 - 1546862。]
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