Schaffer F L, Soergel M E
J Virol. 1976 Sep;19(3):925-31. doi: 10.1128/JVI.19.3.925-931.1976.
A calicivirus, San Miguel sea lion virus serotype 4, isolate 15FT, externally labelled with 125I, was shown by gel electrophoresis to possess a single major polypeptide. The polypeptide migrated anomalously upon electrophoresis in two sodium dodecyl sulfate (SDS) systems: more slowly than bovine serum albumin in a continuous phosphate-buffered system and more rapidly than bovine serum albumin in a discontinuous system. Estimated molecular weights in the two systems were approximately 71,000 and 64,000, respectively. There was no clear evidence for a minor virion polypeptide. Treatment of purified San Miguel sea lion virions with dimethyl suberimidate, a cross-linking reagent, preserved virion integrity during long-term storage at 4 degrees C. Oligomeric species of the polypeptide were observed upon electrophoresis of products from cross-linked virions. Based upon a preferred polypeptide molecular weight estimate of 71,000 and distribution of oligomeric species, a calicivirion model with 120 monomeric protein units is proposed as an alternative to a 180-unit model.
一种杯状病毒，圣米格尔海狮病毒4型，分离株15FT，用¹²⁵I进行外部标记，经凝胶电泳显示具有一条主要的多肽。该多肽在两种十二烷基硫酸钠（SDS）系统中电泳时迁移异常：在连续磷酸盐缓冲系统中比牛血清白蛋白迁移得慢，在不连续系统中比牛血清白蛋白迁移得快。在这两种系统中估计的分子量分别约为71,000和64,000。没有明确证据表明存在次要的病毒粒子多肽。用交联剂辛二酸二甲酯处理纯化的圣米格尔海狮病毒粒子，在4℃长期储存期间保持了病毒粒子的完整性。对交联病毒粒子的产物进行电泳时观察到该多肽的寡聚体种类。基于71,000的首选多肽分子量估计值和寡聚体种类的分布，提出了一种具有120个单体蛋白单元的杯状病毒粒子模型，作为180单元模型的替代方案。
