Masuta C, Ueda S, Suzuki M, Uyeda I
Plant Virology Laboratory, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10487-92. doi: 10.1073/pnas.95.18.10487.
Cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) belong to the Cucumovirus genus. They have a tripartite genome consisting of single-stranded RNAs, designated 1, 2, and 3. Previous studies have shown that viable pseudorecombinants could be created in vitro by reciprocal exchanges between CMV and TAV RNA 3, but exchanges of RNAs 1 and 2 were replication deficient. When we coinoculated CMV RNAs 2 and 3 along with TAV RNAs 1 and 2 onto Nicotiana benthamiana, a hybrid quadripartite virus appeared that consisted of TAV RNA 1, CMV RNAs 2 and 3, and a distinctive chimeric RNA originating from a recombination between CMV RNA 2 and the 3'-terminal 320 nucleotides of TAV RNA 2. This hybrid arose by means of segment reassortment and RNA recombination to produce an interspecific hybrid with the TAV helicase subunit and the CMV polymerase subunit. To our knowledge, this is the first report demonstrating the evolution of a new plant or animal virus strain containing an interspecific hybrid replicase complex.
黄瓜花叶病毒（CMV）和番茄不孕病毒（TAV）属于黄瓜花叶病毒属。它们具有由单链RNA组成的三分体基因组，分别命名为1、2和3。先前的研究表明，通过CMV和TAV RNA 3之间的相互交换可以在体外产生有活力的假重组体，但RNA 1和2的交换在复制方面存在缺陷。当我们将CMV RNA 2和3与TAV RNA 1和2一起接种到本氏烟草上时，出现了一种杂种四分体病毒，它由TAV RNA 1、CMV RNA 2和3以及一种独特的嵌合RNA组成，该嵌合RNA源自CMV RNA 2与TAV RNA 2 3'末端320个核苷酸之间的重组。这种杂种是通过片段重配和RNA重组产生的，形成了一种具有TAV解旋酶亚基和CMV聚合酶亚基的种间杂种。据我们所知，这是第一份证明含有种间杂种复制酶复合体的新植物或动物病毒株进化的报告。
