小鼠胚胎干细胞中Tc1/类水手元件的染色体转座
Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells.
作者信息
Luo G, Ivics Z, Izsvák Z, Bradley A
机构信息
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10769-73. doi: 10.1073/pnas.95.18.10769.
Abstract
Mouse has become an increasingly important organism for modeling human diseases and for determining gene function in a mammalian context. Unfortunately, transposon-tagged mutagenesis, one of the most valuable tools for functional genomics, still is not available in this organism. On the other hand, it has long been speculated that members of the Tc1/mariner-like elements may be less dependent on host factors and, hence, can be introduced into heterologous organisms. However, this prediction has not been realized in mice. We report here the chromosomal transposition of the Sleeping Beauty (SB) element in mouse embryonic stem cells, providing evidence that it can be used as an in vivo mutagen in mice.
摘要
小鼠已成为用于模拟人类疾病和在哺乳动物背景下确定基因功能的越来越重要的生物体。不幸的是，转座子标签诱变作为功能基因组学最有价值的工具之一，在这种生物体中仍然不可用。另一方面，长期以来人们一直推测，Tc1/水手样元件的成员可能较少依赖宿主因子，因此可以引入异源生物体中。然而，这一预测在小鼠中尚未实现。我们在此报告了睡美人（SB）元件在小鼠胚胎干细胞中的染色体转座，提供了它可作为小鼠体内诱变剂的证据。
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