Ito Y, Liu S Q, Imanishi Y
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
Biomaterials. 1991 Jul;12(5):449-53. doi: 10.1016/0142-9612(91)90141-v.
Insulin, transferrin and collagen were immobilized on the surface of hydrolysed poly(methyl methacrylate) films. Mouse STO fibroblasts were cultured on the protein-immobilized films. Growth factors remained immobilized without detachment and accelerated cell growth in a more potent manner than free or adsorbed growth factors. Immobilized collagen enhanced the flattening of adhered cells in the early stages of cell adhesion, but did not enhance cell growth significantly.
胰岛素、转铁蛋白和胶原蛋白被固定在水解聚甲基丙烯酸甲酯薄膜表面。将小鼠STO成纤维细胞培养在固定有蛋白质的薄膜上。生长因子保持固定状态而不脱落，并且比游离或吸附的生长因子更有效地促进细胞生长。固定化的胶原蛋白在细胞黏附早期增强了黏附细胞的扁平化，但对细胞生长的促进作用不显著。
