基于随机扩增多态性DNA（RAPD）标记构建的小菜蛾赤眼蜂复合遗传图谱。
A composite genetic map of the parasitoid wasp Trichogramma brassicae based on RAPD markers.
作者信息
Laurent V, Wajnberg E, Mangin B, Schiex T, Gaspin C, Vanlerberghe-Masutti F
机构信息
Laboratoire de Biologie des Invertébrés, Biologie des Populations, INRA, 06606 Antibes, France.
出版信息
Genetics. 1998 Sep;150(1):275-82. doi: 10.1093/genetics/150.1.275.
Abstract
Three linkage maps of the genome of the microhymenopteran Trichogramma brassicae were constructed from the analysis of segregation of random amplified polymorphic DNA markers in three F2 populations. These populations were composed of the haploid male progeny of several virgin F1 females, which resulted from the breeding of four parental lines that were nearly fixed for different random amplified polymorphic DNA markers and that were polymorphic for longevity and fecundity characters. As the order of markers common to the three mapping populations was found to be well conserved, a composite linkage map was constructed. Eighty-four markers were organized into five linkage groups and two pairs. The mean interval between two markers was 17.7 cM, and the map spanned 1330 cM.
摘要
通过对三个F2群体中随机扩增多态性DNA标记的分离分析，构建了微小膜翅目昆虫甘蓝赤眼蜂基因组的三个连锁图谱。这些群体由几只未交配F1雌蜂的单倍体雄性后代组成，这些F1雌蜂是由四个亲本系杂交产生的，这四个亲本系对不同的随机扩增多态性DNA标记几乎是固定的，并且在寿命和繁殖力性状上具有多态性。由于发现三个作图群体共有的标记顺序保存良好，因此构建了一个复合连锁图谱。84个标记被组织成5个连锁群和2对。两个标记之间的平均间隔为17.7 cM，图谱跨度为1330 cM。
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