腐败厌氧菌3679孢子萌发过程中的事件顺序。
Sequence of events during germination of putrefactive anaerobe 3679 spores.
作者信息
Uehara M, Frank H A
出版信息
J Bacteriol. 1967 Sep;94(3):506-11. doi: 10.1128/jb.94.3.506-511.1967.
Abstract
The sequence of changes during germination of putrefactive anaerobe 3679h spores was studied under aerobic conditions in a solution containing l-alanine and sodium pyrophosphate. Evidence that specific changes occurred in two distinct regions of the spore is given by data on several criteria that were used to measure germination. During the initial stage of germination, the absorbancy decreased, dipicolinic acid was released, the spores lost their resistance to heat and toxic chemicals, and the spore periphery (cortex) darkened gradually under phase-contrast microscopy. The final stage of germination was characterized by changes in the central spore region (core), notably phase darkening of the spore center and stainability with mercurochrome, and by a slight additional absorbancy decrease.
摘要
在含有L-丙氨酸和焦磷酸钠的溶液中，在有氧条件下研究了腐败厌氧菌3679h孢子萌发过程中的变化序列。用于测量萌发的几个标准的数据表明，孢子的两个不同区域发生了特定变化。在萌发初期，吸光度下降，吡啶二羧酸释放，孢子失去对热和有毒化学物质的抗性，并且在相差显微镜下孢子外围（皮层）逐渐变黑。萌发的最后阶段的特征是孢子中心区域（核心）发生变化，特别是孢子中心的相变暗和用汞溴红染色，以及吸光度略有额外下降。
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