细菌芽孢萌发的承诺。触发反应的一种度量。
Commitment of bacterial spores to germinate. A measure of the trigger reaction.
作者信息
Stewart G S, Johnstone K, Hagelberg E, Ellar D J
出版信息
Biochem J. 1981 Jul 15;198(1):101-6. doi: 10.1042/bj1980101.
Abstract
The rate of commitment of bacterial spores to germinate after short exposure to L-alanine increases exponentially from the time of addition of L-alanine. This absence of a lag facilitates kinetic analysis and allows the dependence of commitment on temperature and pH to be determined. The pH profile of commitment has been compared with that obtained from measurements of absorbance decreases during germination, and the two profiles exhibit differing pK values. It is suggested that because the decrease in A600 of spore suspensions is a late event in germination, it is an unsuitable parameter for studying germination-triggering reactions. Commitment has been shown to be temperature-dependent, with an optimum at approx. 37 degrees C and an activation energy (mu) of 1.08 X 10(5) J/mol. The data obtained from the present studies have been used to develop a model for the triggering of germination.
摘要
短时间暴露于L-丙氨酸后，细菌芽孢开始萌发的速率从添加L-丙氨酸之时起呈指数增加。不存在延迟有利于动力学分析，并能确定开始萌发对温度和pH的依赖性。已将开始萌发的pH曲线与萌发过程中吸光度降低的测量结果所得到的曲线进行比较，这两条曲线呈现出不同的pK值。有人提出，由于芽孢悬液在600nm处吸光度的降低是萌发过程中的一个后期事件，因此它不是研究萌发触发反应的合适参数。已证明开始萌发取决于温度，最适温度约为37℃，活化能（μ）为1.08×10⁵J/mol。从本研究中获得的数据已用于建立一个萌发触发模型。
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