Johnstone K, Stewart G S, Scott I R, Ellar D J
Biochem J. 1982 Nov 15;208(2):407-11. doi: 10.1042/bj2080407.
Zinc release is the first quantitatively significant event detected during the triggering of Bacillus megaterium KM spore germination. Of the total spore Zn2+ pool 25% is released from non-heat-activated spores within 4 min of triggering germination. During this period only 10% of the spore population becomes irreversibly committed to germinate. The investigation of a putative role for Zn2+ in the germination trigger mechanism has established a relationship between the rate and extent of Zn2+ release and the stimulation of spore germination by heat activation. Furthermore, a correlation can be demonstrated between the extent of zinc release from spore populations and the time required to obtain 50% commitment of these populations to germinate over a wide temperature range. These findings have been used to expand a recently published model for the triggering of bacterial spore germination.
锌的释放是在巨大芽孢杆菌KM芽孢萌发触发过程中检测到的第一个具有定量意义的重要事件。在触发萌发的4分钟内，25%的总芽孢锌离子库从非热激活芽孢中释放出来。在此期间，只有10%的芽孢群体不可逆地开始萌发。对锌离子在萌发触发机制中假定作用的研究建立了锌离子释放的速率和程度与热激活对芽孢萌发的刺激之间的关系。此外，在很宽的温度范围内，可证明芽孢群体锌释放的程度与这些群体达到50%萌发所需时间之间存在相关性。这些发现已被用于扩展最近发表的细菌芽孢萌发触发模型。
