Moskvina E, Schüller C, Maurer C T, Mager W H, Ruis H
Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Austria.
Yeast. 1998 Aug;14(11):1041-50. doi: 10.1002/(SICI)1097-0061(199808)14:11<1041::AID-YEA296>3.0.CO;2-4.
Stress response elements (STREs, core consensus AG4 or C4T) have been demonstrated previously to occur in the upstream region of a number of genes responsive to induction by a variety of stress signals. This stress response is mediated by the homologous transcription factors Msn2p and Msn4p, which bind specifically to STREs. Double mutants (msn2 msn4) deficient in these transcription factors have been shown to be hypersensitive to severe stress conditions. To obtain a more representative overview of the set of yeast genes controlled via this regulon, a computer search of the Saccharomyces cerevisiae genome was carried out for genes, which, similar to most known STRE-controlled genes, exhibit at least two STREs in their upstream region. In addition to the great majority of genes previously known to be controlled via STREs, 69 open reading-frames were detected. Expression patterns of a set of these were examined by grid filter hybridization, and 14 genes were examined by Northern analysis. Comparison of the expression patterns of these genes demonstrates that they are all STRE-controlled although their detailed expression patterns differ considerably.
应激反应元件（STREs，核心共有序列AG4或C4T）先前已被证明存在于许多受多种应激信号诱导的基因的上游区域。这种应激反应由同源转录因子Msn2p和Msn4p介导，它们特异性结合STREs。已证明缺乏这些转录因子的双突变体（msn2 msn4）对严重应激条件高度敏感。为了更全面地了解通过该调控子控制的酵母基因集，对酿酒酵母基因组进行了计算机搜索，以寻找那些与大多数已知的受STRE控制的基因类似，在其上游区域至少有两个STRE的基因。除了绝大多数先前已知受STREs控制的基因外，还检测到69个开放阅读框。通过网格滤膜杂交检查了其中一组基因的表达模式，并通过Northern分析检查了14个基因。这些基因表达模式的比较表明，它们均受STRE控制，尽管其详细的表达模式差异很大。
