Taylor R N, Fulford K M, Huong A Y
J Clin Microbiol. 1978 Jul;8(1):23-7. doi: 10.1128/jcm.8.1.23-27.1978.
Proficiency testing results were used to compare the kinetic and end-point versions of the single radial immunodiffusion method for quantitating human serum immunoglobulins. Statistical analysis of the results indicated that the results were not normally distributed but that the log normal distribution gives the best fit of any of the well-known frequency distributions. Consequently, statistical analysis of immunoglobulin results must be log transformed before parametric statistical tests can be appropriately applied. In general, there were no significant differences in level, precision, or interlaboratory comparability for these two methods. However, levels were different for participants using Hyland reagents, and better interlaboratory comparability was achieved by the end-point assay for immunoglobulin M. There were no significant differences in reported levels when compared by manufacturer within the same method.
能力验证结果用于比较定量人血清免疫球蛋白的单径向免疫扩散法的动力学版本和终点版本。结果的统计分析表明，结果并非呈正态分布，而是对数正态分布最符合任何一种著名的频率分布。因此，在适当应用参数统计检验之前，必须对免疫球蛋白结果进行对数转换。总体而言，这两种方法在水平、精密度或实验室间可比性方面没有显著差异。然而，使用Hyland试剂的参与者的水平有所不同，免疫球蛋白M的终点测定法在实验室间可比性方面表现更好。在同一方法内按制造商进行比较时，报告的水平没有显著差异。
