Hou X G, Kawamura Y, Sultana F, Shu S, Hirose K, Goto K, Ezaki T
Department of Microbiology, Gifu University School of Medicine, Japan.
Int J Syst Bacteriol. 1998 Apr;48 Pt 2:423-9. doi: 10.1099/00207713-48-2-423.
Three strains of creatinine-hydrolysing bacteria isolated from human urine were characterized taxonomically. They were aerobic, non-spore-forming, Gram-positive rods with the peptidoglycan of the cell wall containing lysine. MK-8 and MK-9 were found to be the major types of menaquinone. The G + C content of the DNA was 66-67 mol%. The 16S rRNA sequence of one strain (GIFU 12498) was determined and aligned with other high-G + C-content Gram-positive rods from different genera. Following phylogenetic analysis, this strain was placed in the genus Arthrobacter. Arthrobacter protophormiae was the most closely related species in the phylogenetic tree, and this species also showed the highest sequence homology value (97%) with GIFU 12498. However, DNA-DNA hybridization indicated that GIFU 12498 did not belong to A. protophormiae (33.8 +/- 3.5% chromosomal similarity). The three urine strains belonged to one species because they shared more than 95% DNA-DNA similarity. It is proposed that these strains are placed in the genus Arthrobacter as a new species, Arthrobacter creatinolyticus sp. nov. The type strain of A. creatinolyticus is GIFU 12498, which has been deposited in the Japan Collection of Microorganisms (JCM) with the accession number JCM 10102.
对从人尿液中分离出的三株肌酐水解细菌进行了分类学鉴定。它们为需氧菌，无芽孢，革兰氏阳性杆菌，细胞壁肽聚糖含赖氨酸。发现MK - 8和MK - 9是主要的甲基萘醌类型。DNA的G + C含量为66 - 67摩尔%。测定了其中一株（GIFU 12498）的16S rRNA序列，并与来自不同属的其他高G + C含量革兰氏阳性杆菌进行比对。经过系统发育分析，该菌株被归入节杆菌属。在系统发育树中，原光节杆菌是与之关系最密切的物种，且该物种与GIFU 12498的序列同源性值也最高（97%）。然而，DNA - DNA杂交表明GIFU 12498不属于原光节杆菌（染色体相似性为33.8 +/- 3.5%）。这三株尿液菌株属于同一个物种，因为它们的DNA - DNA相似性超过95%。建议将这些菌株归入节杆菌属，作为一个新物种，即解肌酐节杆菌新种。解肌酐节杆菌的模式菌株是GIFU 12498，已保藏于日本微生物保藏中心（JCM），保藏编号为JCM 10102。
