Lambert L H, Cox T, Mitchell K, Rosselló-Mora R A, Del Cueto C, Dodge D E, Orkand P, Cano R J
XOMA Corporation, Berkeley, CA 94710, USA.
Int J Syst Bacteriol. 1998 Apr;48 Pt 2:511-8. doi: 10.1099/00207713-48-2-511.
Two bacterial isolates, designated AMG-D1T and AMG-D2, were recovered from 25-35-million-year-old Dominican amber. AMG-D1T and AMG-D2 biochemically most closely resemble Staphylococcus xylosus; they differ physiologically from other staphylococci. Fatty acid analysis and comparisons with extensive databases were unable to show relatedness to any specific taxon. Moreover, AMG-D1T and AMG-D2 contain tuberculostearic acid and meso-diaminopimelic acid, characteristic of the G + C-rich coryneform bacteria, as opposed to L-lysine characteristic of staphylococci. AMG-D1T and AMG-D2 have a G + C ratio of 35 mol%. Phylogenetic analysis with the 16S rRNA gene indicated that AMG-D1T and AMG-D2 were most closely related to Staphylococcus equorum, S. xylosus, Staphylococcus saprophyticus and other novobiocin-resistant staphylococci. Stringent DNA-DNA hybridization studies with AMG-D1T revealed similarities of 38% with S. equorum, 23% with S. xylosus and 6% with S. saprophyticus. The results indicate that AMG-D1T and AMG-D2 represent a novel species, which was named Staphylococcus succinus sp. nov. The type strain of the new species is AMG-D1 (ATCC 700337).
从距今2500万至3500万年的多米尼加琥珀中分离出了两株细菌，分别命名为AMG-D1T和AMG-D2。AMG-D1T和AMG-D2在生化特性上与木糖葡萄球菌最为相似；它们在生理特征上与其他葡萄球菌不同。脂肪酸分析以及与大量数据库的比对均无法显示其与任何特定分类单元的相关性。此外，AMG-D1T和AMG-D2含有结核硬脂酸和内消旋二氨基庚二酸，这是富含G + C的棒状杆菌的特征，而非葡萄球菌特有的L-赖氨酸。AMG-D1T和AMG-D2的G + C含量为35 mol%。基于16S rRNA基因的系统发育分析表明，AMG-D1T和AMG-D2与马胃葡萄球菌、木糖葡萄球菌、腐生葡萄球菌以及其他耐新生霉素葡萄球菌关系最为密切。对AMG-D1T进行的严格DNA-DNA杂交研究显示，其与马胃葡萄球菌的相似度为38%，与木糖葡萄球菌的相似度为23%，与腐生葡萄球菌的相似度为6%。结果表明，AMG-D1T和AMG-D2代表一个新物种，命名为琥珀葡萄球菌（Staphylococcus succinus sp. nov.）。该新物种的模式菌株为AMG-D1（ATCC 700337）。
