马铃薯X病毒三基因块在体内的翻译需要两种亚基因组mRNA。
In vivo translation of the triple gene block of potato virus X requires two subgenomic mRNAs.
作者信息
Verchot J, Angell S M, Baulcombe D C
机构信息
Sainsbury Laboratory, Norwich Research Park, Colney, United Kingdom NR4 7UH.
出版信息
J Virol. 1998 Oct;72(10):8316-20. doi: 10.1128/JVI.72.10.8316-8320.1998.
Abstract
The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an in vivo complementation assay, we have shown that the 25K protein is expressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.
摘要
马铃薯X病毒的25千道尔顿（25K）、12K和8K运动蛋白源自重叠的开放阅读框（ORF）。通过体内互补试验，我们已经表明25K蛋白由功能上的单顺反子mRNA表达，而12K和8K蛋白则来自双顺反子mRNA。8K ORF的翻译是通过核糖体渗漏扫描12K ORF实现的。
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本文引用的文献
1
Molecular cloning of clover yellow mosaic virus RNA: identification of coat protein coding sequences in vivo and in vitro.
Virology. 1987 Apr;157(2):276-84. doi: 10.1016/0042-6822(87)90270-4.
2
Complementation of a potato virus X mutant mediated by bombardment of plant tissues with cloned viral movement protein genes.
J Gen Virol. 1997 Aug;78 ( Pt 8):2077-83. doi: 10.1099/0022-1317-78-8-2077.
3
Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA.在表达复制型马铃薯X病毒RNA的转基因植物中实现持续的基因沉默。
EMBO J. 1997 Jun 16;16(12):3675-84. doi: 10.1093/emboj/16.12.3675.
4
Mutation of the GKS motif of the RNA-dependent RNA polymerase from potato virus X disables or eliminates virus replication.马铃薯X病毒依赖RNA的RNA聚合酶的GKS基序发生突变会使病毒复制失效或消除。
J Gen Virol. 1997 Jun;78 ( Pt 6):1247-51. doi: 10.1099/0022-1317-78-6-1247.
5
Translation in plants--rules and exceptions.植物中的翻译——规则与例外
Plant Mol Biol. 1996 Oct;32(1-2):159-89. doi: 10.1007/BF00039382.
6
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J Virol. 1996 Aug;70(8):5533-40. doi: 10.1128/JVI.70.8.5533-5540.1996.
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8
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Mol Gen Genet. 1993 Apr;238(1-2):59-64. doi: 10.1007/BF00279531.
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Crit Rev Biochem Mol Biol. 1993;28(5):375-430. doi: 10.3109/10409239309078440.
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