人EB病毒相关胃癌在SCID小鼠中的建立与特性研究
Establishment and characterization of a human Epstein-Barr virus-associated gastric carcinoma in SCID mice.
作者信息
Iwasaki Y, Chong J M, Hayashi Y, Ikeno R, Arai K, Kitamura M, Koike M, Hirai K, Fukayama M
机构信息
Departments of Surgery, Tokyo Metropolitan Komagome Hospital, Tokyo, Japan.
出版信息
J Virol. 1998 Oct;72(10):8321-6. doi: 10.1128/JVI.72.10.8321-8326.1998.
Abstract
A transplantable human Epstein-Barr virus-associated gastric carcinoma (EBVaGC), designated KT, was propagated in severe combined immunodeficiency (SCID) mice for 12 passages. Mucin and cytokeratin expression and the Alu sequence in tumor DNA confirmed that the KT tumor was derived from human epithelial tissue. The identity of clonal EBV in the original and KT tumors was demonstrated by terminal repeat analysis of EBV DNA. The pattern of latency gene expression of EBV was the same in both tumors. EBER1 was presented similarly in tumor cell nuclei by in situ hybridization. Reverse transcription-PCR analysis also demonstrated Q-promoter-driven EBNA1 expression but not BZLF1, EBNA2, or LMP1 expression. Thus, the transplantable human EBVaGC KT retains the original EBV with the same latency gene expression and can serve as a model for this unique type of gastric carcinoma.
摘要
一种可移植的人类爱泼斯坦-巴尔病毒相关胃癌（EBVaGC），命名为KT，在严重联合免疫缺陷（SCID）小鼠中传代培养了12代。肿瘤DNA中的粘蛋白和细胞角蛋白表达以及Alu序列证实KT肿瘤源自人类上皮组织。通过对EBV DNA的末端重复序列分析，证明了原始肿瘤和KT肿瘤中克隆性EBV的一致性。两种肿瘤中EBV潜伏基因表达模式相同。原位杂交显示EBER1在肿瘤细胞核中的呈现方式相似。逆转录聚合酶链反应分析也表明存在Q启动子驱动的EBNA1表达，但不存在BZLF1、EBNA2或LMP1表达。因此，可移植的人类EBVaGC KT保留了具有相同潜伏基因表达的原始EBV，可作为这种独特类型胃癌的模型。
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