Esterbauer H
Institute of Biochemistry, University of Graz, Austria.
Pathol Biol (Paris). 1996 Jan;44(1):25-8.
Numerous methods have been developed to measure lipid peroxidation products and lipid peroxidation damage in tissues, cells and body fluids. The choice of which method is most appropriate depends, amongst others, on the specific interest of the investigator. In the routine clinical and laboratory praxis, the determination of thiobarbituric acid reactive substances (TBARS) under strictly standardized conditions is in most cases the first choice. The specificity of the colorimetric or fluorimetric assay can be significantly improved if combined with HPLC. If levels of TBARS are increased, other more sophisticated assays should be performed for verification. Assays are available for: Phospholipid- and cholesterylester hydroperoxides, aldehydic lipid peroxidation products including 4-hydroxynonenal, fluorescent protein adducts (e.g. lipofuscin), conjugated dienes and antioxidants. The measurement of pentane and ethane in the exhaled air by gas chromatography is the only available non-invasive method. Several laboratories currently develop immunological assays (ELISA, RIA) for determining proteins modified by lipid peroxidation products (e.g. malonaldehyde, 4-hydroxynonenal) or autoantibodies against oxidatively modified proteins. It can be expected that such assays will soon gain diagnostic importance.
已经开发出许多方法来测量组织、细胞和体液中的脂质过氧化产物以及脂质过氧化损伤。选择哪种方法最合适，除其他因素外，取决于研究者的具体兴趣。在常规临床和实验室实践中，在严格标准化条件下测定硫代巴比妥酸反应性物质（TBARS）在大多数情况下是首选。如果与高效液相色谱（HPLC）结合，比色法或荧光法测定的特异性可显著提高。如果TBARS水平升高，则应进行其他更复杂的测定以进行验证。可用于检测的物质包括：磷脂和胆固醇酯氢过氧化物、醛类脂质过氧化产物（包括4-羟基壬烯醛）、荧光蛋白加合物（如脂褐素）、共轭二烯和抗氧化剂。通过气相色谱法测量呼出气体中的戊烷和乙烷是唯一可用的非侵入性方法。目前有几个实验室正在开发免疫测定法（酶联免疫吸附测定法、放射免疫测定法），用于测定被脂质过氧化产物（如丙二醛、4-羟基壬烯醛）修饰的蛋白质或针对氧化修饰蛋白质的自身抗体。可以预期，此类测定法很快将具有诊断重要性。
