Kunathikom S, Ko-Anantakul S, Techatraisak K, Treetampinich C, Phophong P, Karavagul C
Department of Obstetrics and Gynecology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
J Med Assoc Thai. 1998 Sep;81(9):682-7.
To compare the efficiency of sperm preparation between the two-layer Percoll gradient and mini-Percoll methods, 50 normal and 33 abnormal semen samples from male partners of infertile couples were studied. The number of recovered spermatozoa, percentage of motility, percentage of normal morphology, and their survival at 24 and 48 hours were assessed. Both Percoll gradient techniques resulted in a significantly higher percentage of motility and percentage of normal morphology compared with the original semen samples (p < 0.0001). The two-layer Percoll gradient showed a higher sperm recovery than the mini-Percoll method (p < 0.001), but the latter resulted in a higher percentage of motility (p > 0.001) and a higher sperm survival rate at 24 hours (p < 0.05) than the former, regarding normal semen samples. These differences did not appear with abnormal semen samples when analyzed as a group. Considering each of the abnormal parameters separately, sperm recovery was significantly higher after the two-layer Percoll gradient in the case of astheno- and teratozoospermia (p < 0.05), but sperm survival at 48 hours was higher after the mini-Percoll gradient in the case of teratozoospermia (p < 0.05). It is concluded that both the two-layer Percoll gradient and mini-Percoll method can be used effectively for sperm preparation. The former yields a higher sperm recovery, but the latter should be considered regarding teratozoospermic samples and semen samples of very low volume.
为比较两层Percoll梯度法和微量Percoll法在精子处理效率上的差异，对50例来自不育夫妇男性伴侣的正常精液样本和33例异常精液样本进行了研究。评估了回收精子的数量、活动率、正常形态百分比以及它们在24小时和48小时后的存活率。与原始精液样本相比，两种Percoll梯度技术均使活动率百分比和正常形态百分比显著更高（p < 0.0001）。对于正常精液样本，两层Percoll梯度法的精子回收率高于微量Percoll法（p < 0.001），但后者的活动率百分比更高（p > 0.001），且在24小时时精子存活率更高（p < 0.05）。将异常精液样本作为一组分析时，这些差异未出现。分别考虑每个异常参数，在弱精子症和畸形精子症病例中，两层Percoll梯度法后的精子回收率显著更高（p < 0.05），但在畸形精子症病例中，微量Percoll梯度法后的48小时精子存活率更高（p < 0.05）。结论是，两层Percoll梯度法和微量Percoll法均可有效地用于精子处理。前者精子回收率更高，但对于畸形精子症样本和极低体积的精液样本，应考虑使用后者。
