用于检测和鉴定营养缺陷菌属的聚合酶链反应
PCR for detection and identification of Abiotrophia spp.
作者信息
Roggenkamp A, Leitritz L, Baus K, Falsen E, Heesemann J
机构信息
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, 80336 Munich, Germany.
出版信息
J Clin Microbiol. 1998 Oct;36(10):2844-6. doi: 10.1128/JCM.36.10.2844-2846.1998.
Abstract
Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiotrophia spp. were developed. The first PCR assay was positive for all Abiotrophia spp. Subsequently performed restriction fragment length polymorphism analysis allowed the verification of the PCR amplicons and the differentiation of the three species. The second PCR assay was positive only for A. elegans, the most fastidious species of Abiotrophia. Both PCR assays were shown to be specific and sensitive and should facilitate the identification of Abiotrophia spp.
摘要
过去被称为营养变异链球菌的阿托菲氏菌属成员，是引起败血症和心内膜炎的重要病原体。阿托菲氏菌属的培养和生化鉴别往往很困难。基于16S rRNA序列，开发了两种用于检测和鉴定阿托菲氏菌属的PCR检测方法。第一种PCR检测方法对所有阿托菲氏菌属均呈阳性。随后进行的限制性片段长度多态性分析可验证PCR扩增产物并区分这三个菌种。第二种PCR检测方法仅对阿托菲氏菌属中要求最苛刻的优雅阿托菲氏菌呈阳性。两种PCR检测方法均显示出特异性和敏感性，应有助于阿托菲氏菌属的鉴定。
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